应激时大鼠胃粘膜中MDA含量和CAT、Ca^(2+)-ATPase活性的变化  

Changes in Malonyldialdehyde Content and Activities of Catalase and Ca^(2+)-ATPase in Rat Gastric Mucosa under Stress

作　　者：许燕[1] 张芸[2] 

机构地区：[1]镇江医学院病理生理学教研室,江苏镇江212001 [2]镇江医学院药理学教研室,江苏镇江212001

出　　处：《南京铁道医学院学报》2000年第4期241-243,共3页Journal of Nanjing Railway Medical College

摘　　要：目的 :探讨在应激状态下大鼠胃粘膜中丙二醛 (MDA)含量以及过氧化氢酶 (CAT)和钙泵 (Ca2 + ATPase)活性的变化。方法 :捆扎大鼠四肢 ,固定头部后淹水制成应激模型 ,检测其胃粘膜中MDA含量、CAT活性和Ca2 + ATpase活性。结果 :随着应激时间的延长 ,胃粘膜损伤加重 ,粘膜中MDA含量逐步增加 ,CAT和Ca2 + ATPase活性也代偿性增加 ,在应激2h组活性较高。结论 :随着应激时间的延长 ,机体产生的自由基大量增加 ,尽管清除系统活性增加 ,但仍不足以及时将大量的自由基清除掉 。Objective This experiment was designed to test the changes in the malonyldialdehyde(MDA) content and the activities of catalase (CAT) and Ca 2+ ATPase in rat gastric mucosa under stress.Methods The animal model was established by soaking the rat in water after binding four limbs and fixing the head on shelf.The level of MDA and the activities CAT and Ca 2+ ATPase in rat gastric mucosa were determined. Results The content of MDA was increased with the duration of stress and the activities of CAT and Ca 2+ ATPase were enhanced compensatively.The peak was found at the second hour after stress.Conclusion The radials increased as stress time went on.Although the activities of radical clearing system enhanced compa^ratively,it could not clear the increasing radicals timely.Thus the injury of rat gastric mucosa occurred.

关 键 词：应激 胃粘膜 丙二醛 过氧化氢酶 钙泵 

分 类 号：R363.2[医药卫生—病理学]