原癌基因Pim-3在胰腺癌组织中的表达及其与胰腺癌细胞增殖的相关性  

Expression of Pim-3 in pancreatic carcinoma and its correlation with cell proliferation

作　　者：陆平[1,2] 李云鹏[1,3] 高岩[1,4] 刘景波[1,2] 赵文杰[1,2] 刘言[1,2] 邢春艳[1,2] 

机构地区：[1]齐齐哈尔医学院附属五院 [2]大庆龙南医院病理科,黑龙江大庆163453 [3]大庆龙南医院妇产科,黑龙江大庆163453 [4]大庆龙南医院营养科,黑龙江大庆163453

出　　处：《癌变．畸变．突变》2014年第1期45-48,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨胰腺癌组织中Pim-3的表达及其与癌细胞增殖的相关性。方法:采用免疫组化SP法检测15例正常胰腺组织和49例胰腺癌组织中Pim-3与增殖细胞核抗原(PCNA)蛋白的表达,并计算PCNA增殖指数。结果:Pim-3在正常胰腺组织中不表达,而在胰腺癌组织中阳性表达,表达率为73.47%(36/49),明显高于正常胰腺组织(P<0.01)。胰腺癌组织中PCNA的增殖指数(32.54%±14.69%)明显高于正常胰腺组织(6.25%±2.59%)(P<0.01)。且随胰腺癌细胞中Pim-3蛋白表达水平升高,肿瘤细胞增殖活性相应增加,Pim-3蛋白表达与PCNA增殖指数呈正相关(r=0.726,P<0.01)。结论:Pim-3在胰腺癌组织中高表达,Pim-3与细胞的增殖密切相关,可能在胰腺癌的发生发展中起重要作用。OBJECTIVE：To investigate the expression of Pim-3 in pancreatic carcinoma and its correlation with cell proliferation. METHODS：The streptavidin-peroxidase complex technique was used to examine the expression of Pim-3 and PCNA proteins in 15 normal pancreatic tissue and 49 pancreatic carcinoma and the proliferative index （PI） was calculated. RESULTS：In normal pancreatic tissue Pim-3 was not detected,whereas in pancreatic carcinoma the positive expression ratio of Pim-3 was 73.47% （36/49）.The positive rate of Pim-3 in pancreatic carcinoma was significantly higher than that in normal pancreatic tissue （P〈0.01）.The PI in normal pancreatic tissue and pancreatic duct adenocarcinoma was 6.25%±2.59% and 32.54%±14.69%,respectively,also with a significant difference （P〈0.01）.With the increase in the expression of Pim-3 protein,the cell proliferating activity increased in the tumor cells. The Pim-3 expression level was positively correlated with PI （r=0.726,P〈0.01）. CONCLUSION：Pim-3 was overexpresed in human pancreatic carcinoma,and it may play important roles in the carcinogenesis and progression of pancreatic carcinoma.

关 键 词：胰腺癌 PIM-3 增殖细胞核抗原 

分 类 号：R736.7[医药卫生—肿瘤]