乙醇诱导小鼠急性肝细胞损伤模型中NF-κB的表达  

Expression of nuclear factorkappa B in mouse model of alcohol-induced acute hepatocyte injury

作　　者：何宁[1] 张静姝[1] 张静[1] 姜淑卿[1] 刘英华[1] 管彤[1] 李新[1] 

机构地区：[1]天津市疾病预防控制中心毒理室,天津300011

出　　处：《癌变．畸变．突变》2014年第1期52-55,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：天津市卫生局科技基金(06KZ65)

摘　　要：目的:研究核因子NF-κB在乙醇诱导小鼠急性肝细胞损伤中的表达及意义。方法:40只小鼠随机分成对照组和3个乙醇(250、500、1 000 mmol/kg)染毒组,每组10只。一次性灌胃染毒,16 h后检测小鼠血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)的活力;染毒24 h后采用HE及油红O染色进行病理学观察;采用免疫组织化学法观察小鼠肝细胞中NF-κB蛋白的表达。结果:3个乙醇染毒组小鼠血清中的转氨酶AST和ALT活力均高于对照组(P均<0.05);HE染色发现小鼠肝组织中出现肝细胞内空泡变性。油红O染色显示损伤的肝细胞内出现大量脂滴。免疫组织化学检测发现500和1 000 mmol/kg乙醇染毒组小鼠肝细胞胞质及胞核内NF-κB蛋白表达的积分光密度明显高于对照组(P<0.05)。结论:成功复制了急性小鼠酒精性肝损伤的模型,NF-κB升高是小鼠急性酒精性肝细胞损伤的一个重要分子事件。OBJECTIVE：To investigate the expression and significance of nuclear factor-κappa B in alcohol-induced acute hepatocyte injury. METHODS：Forty mice were randomly divided into a control group and three alcohol treated groups （250,500,1 000 mmol/kg）. Each group included ten mice. 16 h after gavage,serum aspartate aminotransferase （AST） and alanine aminotransferase （ALT） levels were measured. After 24 h,the liver was examined by HE staining and oil Red O staining. The expression of NF-κB protein in hepatocytes was assessed by immunohistochemistry. RESULTS：Serum AST and ALT levels in three alcohol-treated groups were significantly increased compared with that in the control group （P〈0.05）. There was vacuolar degeneration in hepatocytes by HE staining. There were large amounts of Oil Red O staining droplets in the hepatocytes. In alcohol-treated groups （500, 1 000 mmol/kg）,the integrated optical density expression of NF-κB protein in hepatocytes cytoplasm and nucleus were significantly higher （P〈0.05）. CONCLUSION：This study successfully set up an alcohol-induced acute hepatocyte injury mouse model,and illustrated the role of NF-κB in the development and progression of acute alcoholic-induced hepatocyte injury.

关 键 词：NF-ΚB 乙醇 肝细胞 损伤 

分 类 号：R363.13[医药卫生—病理学]