漆酶催化麻织物固定化溶菌酶及其抗菌性能  

Immobilization of lysozyme catalyzed by laccase on bast fabrics and its antibacterial property

作　　者：董爱学 王强[1] 范雪荣[1] 王平[1] 崔莉[1] 袁久刚[1] 章金芳 

机构地区：[1]江南大学生态纺织教育部重点实验室,江苏无锡214122 [2]新天龙集团有限公司,浙江上虞312369

出　　处：《功能材料》2014年第2期44-48,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(51173071);教育部高等学校博士学科点专项科研基金资助项目(20110093110003);教育部长江学者和创新团队发展计划资助项目(IRT1135);江苏省自然科学基金资助项目(BK2011157);江苏省"六大人才高峰"资助项目(2011-XCL-002);江苏高校优势学科建设工程资助项目(苏政办发2011-137号)

摘　　要：利用漆酶对木质素的催化作用将溶菌酶接枝到富含木质素的麻织物上,赋予其抗菌性。通过考马斯亮蓝染色法证明了溶菌酶的接枝,对两种不同木质素含量的织物(棉/亚麻混纺织物、黄麻织物)的接枝效果进行了比较,并应用扫描电镜(SEM)考察了接枝后麻纤维表面形态,研究了溶菌酶固定化后麻织物的抗菌性能和耐洗稳定性。结果表明,麻织物经漆酶催化可实现溶菌酶的固定化,麻纤维木质素含量越高,溶菌酶接枝越多,整理后棉/亚麻织物和黄麻织物抑菌率分别达到60.38%和72.87%,且通过接枝法固定溶菌酶的麻织物较吸附固定法具有更好的耐水洗稳定性和抗菌效果。Grafting of lysozyme catalyzed by laccase on bast fabrics was studied.The antibacterial properties of fabrics were obtained.The graft of lysozyme was proved by method of dyeing with coomassie brilliant blue.The grafting effects of two kinds of fabrics with different lignin content （cotton/flax fabric and j ute fabric）were compared.Scanning electron microscopy （SEM）presented the surface morphologies of grafted bast fibers.The washing stability and the an-tibacterial property of lysozyme immobilized bast fabrics were determined.The results showed that lysozyme can be im-mobilized on bast fabrics via the catalysis of laccase and the grafting level was gradually increasing with the increase in lignin content of bast fabrics.The antibacterial ratios of cotton/flax fabric and jute fabric could reach 60.38% and 72.87% respectively.The fabrics lysozyme immobilized by grafting would obtain better washing stability and antibacte-rial property than that immobilized only by adsorption.

关 键 词：溶菌酶 固定化 漆酶 抗菌 麻织物 

分 类 号：TS195[轻工技术与工程—纺织化学与染整工程]