褪黑素对小鼠MFC前胃癌细胞ERK、Akt及NF-κB表达的影响  

Effects of melatonin on ERK,Akt and NF-κB expressions in murine foregastric cancer cells

作　　者：徐丽[1,2] 宫喜[1] 刘卉[1] 宋军[1] 周瑞祥[1] 

机构地区：[1]福建医科大学人体解剖学与组织胚胎学系神经生物学研究中心,福州350004 [2]莆田学院基础医学院生理学教研室,莆田351100

出　　处：《中国组织化学与细胞化学杂志》2014年第1期1-5,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(30971541);福建省科技厅重点项目(2012Y0033);福建省自然科学基金资助(2012J01411);莆田市科技计划项目(2012S11(2))

摘　　要：目的探讨褪黑素对小鼠MFC前胃癌细胞ERK、Akt及NF-κB表达的影响。方法建立褪黑素在不同时间点干预胃癌细胞的体外模型,免疫印迹法观察褪黑素对小鼠MFC前胃癌细胞p-ERK/ERK、p-Akt/Akt,NF-κB表达的影响,CCK-8检测PI3K抑制剂Wortmannin对MFC细胞增殖的作用。结果①2mmol/L褪黑素作用24h、48h后明显下调MFC细胞ERK1/2、Akt的磷酸化,但对ERK1/2、Akt表达无影响;②Wortmannin明显抑制MFC细胞增殖活性,与MLT作用有明显协同效应;③褪黑素对NF-κB表达无影响。结论褪黑素可通过抑制ERK1/2、Akt的磷酸化从而抑制胃癌细胞增殖。Objective To explore the effects of melatonin on ERK, Akt and NF-κB expressions in murine foregastric cancer （MFC） cells. Methods We established a cell model of MFC cells which were trea ted with melatonin at different time points. The expressions of p ERK/ERK, p Akt/Akt and NF-κB were detected by Western blot. The method of CCK-8 was performed to detect tee effect of Wortmannin, a in hibitor of PI3K, on tee proliferation of MFC cells. Results 1. After 24E and 48h, tee 2mmol/L melatonin significantly down regulated the ERK1/2 and AKT phosphorylation of MFC cells, but had no effect on the expression of ERK1/2 and AKT. 2. Wortmannin significantly inhibited MFC cell proliferation, and had obvious synergistic effect to MLT. 3. Melatonin had no effect on the expression of NF-κB. Conclusion Me latonin can inhibit phosphorylation of ERK1/2 and Akt, thereby inhibiting gastric cancer cell proliferation.

关 键 词：褪黑素 小鼠MFC胃癌细胞 AKT ERK12 NF-ΚB 

分 类 号：R735.2[医药卫生—肿瘤]