多黏菌素B发酵培养基的优化  

Optimization of Culture Medium for Polymyxin B Production

作　　者：马川川[1] 魏晓东[1] 赵波[1] 那可[1] 赵文杰[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国医药工业杂志》2014年第6期531-533,共3页Chinese Journal of Pharmaceuticals

摘　　要：本研究在摇瓶水平上优化了多黏芽孢杆菌(Bacillus polymyxa)的发酵培养基,以提高多黏菌素B的产量。采用单因素试验法和响应面法优化发酵培养基配方,并在7 L发酵罐中进行验证。优化后的发酵培养基配方为全麦粉6.7%、玉米浆粉0.3%、葡萄糖0.5%、硫酸铵0.9%、磷酸二氢钾0.03%和碳酸钙0.03%。此条件下,多黏菌素B的摇瓶发酵产量为0.95 g/L,较优化前提高120.9%,7 L发酵罐中的产量为1.06 g/L。A single factor experiment and response surface methodology were employed for the optimization of culture medium for polymyxin B production from Bacillus polymyxa.The optimum culture medium was verified on the 7 L fermentation tank as follows：wheat flour of 6.7 ％,corn steep powder of 0.3 ％,glucose of 0.48 ％,ammonium sulfate of 0.9％,monopotassium phosphate of 0.03％ and calcium carbonate of 0.03 ％.Under such conditions,the yield of polymyxin B was 0.95 g/L in shaking flasks,120.9％ higher than the original,and it was 1.06 g/L in the 7 L fermentation tank.

关 键 词：多黏菌素B 培养基优化 响应面法 发酵 

分 类 号：TQ465[化学工程—制药化工]