膀胱移行细胞癌中p16、p53及cerbB2蛋白的异常表达及临床意义  

p16,p53 and c-erbB-2 gene expression in bladder carcinoma.

作　　者：朱怀甫[1] 任瑞霞[2] 马群[3] 司传平[3] 陈廷[4] 

机构地区：[1]山东济宁医学院实验动物中心,272013 [2]山东济宁市第一人民医院血液病理科 [3]济宁医学院免疫学教研室 [4]济宁医学院微生物学教研室

出　　处：《中国优生与遗传杂志》2000年第6期13-15,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的 :研究膀胱癌组织中 p16、p5 3及c erbB 2蛋白表达 ,探讨其与膀胱癌病理分级、临床分期和转移的关系。方法 :应用免疫组化SP法对 75例膀胱癌组织中p16、p5 3及c erbB 2蛋白表达进行检测。结果 :75例膀胱癌中 p16、p5 3及c erbB 2的阳性率分别为 41 3 % (31/ 75 )、44 % (33/ 75 )和 40 % (30 / 75 ) ,p16、和c erbB 2基因在膀胱癌中的阳性率与肿瘤病理分级和临床分期有显著性统计学意义 (P <0 .0 5 ) ,p5 3及c erbB 2阳性率与肿瘤临床分期及转移有密切的关系 (P <0 .0 1) .77.3% (5 8/ 75 )肿瘤有上述癌基因和 (或 )抑癌基因的异常表达 ,其中 5 3 .3 % (4 0 / 75 )的肿瘤同时有多个基因的表达异常。结论 :肿瘤的多基因分析比单基因分析更有价值 ,癌基因c erbB 2和抑癌基因p16、p5 3基因的表达异常及协同作用在膀胱癌的发生发展中起重要作用。Stuby on the expression of p16,p53 and c-erbB-2 protein in bladder carinoma and their correlation with the tumor grade,clinic stage and metastasis of bladder carcinoma.Methods:Immunohistochemical method was used to detect the p16,p53 and c-erbB-2 gene expression in 75 cases of bladder carcinoma.Results:Positive rates of p16,p53 and c-erbB-2 in 75 cases of bladder cancer were 41.3%,44% and 40%,respectively.p16 and c-erbB-2 gene expression were related to the tumor grade and clinic stage (P<0.05),p53 and c-erbB-2 gene expression were related to the clinic stage and metastasis (P<0.01).There were oncogenes and/or antioncogenes altered expression in most bladder carcinoma(77.3%),and oncogenes and /or antioncogenes with multiple altered expression in 53.3%.Conclusion:The analysis of multiple oncogene and/or antioncogene is more valuadle than that of single oncogene and/or antioncogene.The altered expression of oncogene c-erbB-2 and antioncogene p16,p53 might play a promotive role in the tumorigenesis and development of bladder tumors.

关 键 词：膀胱肿瘤 癌 基因 免疫组织化学 

分 类 号：R737.14[医药卫生—肿瘤]