急性肺栓塞家兔血清中细胞因子的变化及其意义  

Changes and significances of serum cytokines in acute pulmonary embolism rabbits

作　　者：杨永[1] 张艳敏[2] 梁明亭[1] 骆峰[1] 武蕾[1] 李义波[1] 

机构地区：[1]山东省聊城市人民医院心内科,山东聊城252000 [2]山东省聊城市人民医院急诊科,山东聊城252000

出　　处：《中国现代医学杂志》2014年第12期12-15,共4页China Journal of Modern Medicine

摘　　要：目的观察急性肺栓塞家兔血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、肝细胞生长因子(HGF)的变化,探讨这三种因子在急性肺栓塞中的意义。方法采用兔自体血栓回输法建立急性肺栓塞模型。选用20只健康新西兰白兔,随机分为对照组、模型组。应用酶联免疫吸附法(ELISA)检测血清中TNF-α、IL-1β、HGF的浓度变化。结果在肺血栓栓塞1 h TNF-α、IL-1β就已经升高;3 h达高峰,此时模型组TNF-α、IL-1β(5.52±0.99)ng/mL,(183.06±30.43)pg/mL明显高于对照组(2.16±0.63)ng/mL,(94.26±8.57)pg/mL,有显著性差异(P<0.01);6 h基本达基线水平。肺栓塞后1 h模型组HGF水平开始逐渐增高;于12 h达到高峰,与同期对照组相比有显著差异(474.24±72.77)pg/mL vs(58.46±14.38)pg/mL(P<0.0001)。肺组织病理变化显示:模型组肺动脉内可见多处致密血栓,一些肺泡萎陷,有一些肺泡水肿,肺泡腔内可见大量炎性细胞浸润和少量红细胞渗出液。对照组兔肺未见上述病理变化。结论急性肺栓塞后,血清TNF-α、IL-1β、HGF明显升高,可能参与了急性肺栓塞的病理生理过程;为临床初步诊断急性肺栓塞提供了一定的参考性指标。【Objective】To observe the changes of serum tumor necrosis factor-α（TNF-α） interleukin1β（IL-1β） Hepatocyte growth factor（HGF） and to explore the significances of them in acute pulmonary embolism（APE） rabbit. 【Methods】APE models were established by injecting autologous blood clots into right atrium by femoral vein. 20 healthy zelanian rabbits were selected and divided into the control group（n =10）, model group（n =10）, randomly. The changes of TNF-α IL-1β HGF in the blood serum were tested with an enzyme-linked immunosorbent assay（ELISA）. When this experiment was over, all of the rabbits were sacrificed, then lungs were taken out for pathological analysis. 【Results】At 1 hour after embolization, blood serum TNF-α IL-1β level significantly increased. At 3 hour, TNF-α IL-1β of model group arrived at（5.52±0.99） ng/mL,（183.06±30.43） pg/mL, respectively. At 6 hour, they returned to baseline levels. Blood serum HGF level gradually increased and reached a peak at 12 h in model group. At 12 hour, HGF level was（474.24±72.77） pg/mL which was significantly higher than（58.46±14.38） pg/mL in control group（P〈0.0001）. Histological changes indicated multi-thrombus was visualized in pulmonary artery. Some pulmonary Alveolus presented collapse and some displayed edematous shapes. A plenty of inflammatory cells infiltrated and a little of red cells leaked were seen in alveolar spaces. Partial pulmonary veins showed congestion phenomena. Above-mentioned changes were not observed in control group. 【Conclusions】After APE, TNF-α IL-1β HGF increased obviously. They may be involved in the pathophysiological process of acute pulmonary embolism and provide a certain reference indicators for the preliminary diagnosis of acute pulmonary embolism.

关 键 词：肺栓塞 动物模型 细胞因子 肝细胞生长因子 

分 类 号：R-332[医药卫生]