大鼠单侧大脑中动脉栓塞后脑内乙酰胆碱酯酶的光镜与电镜酶组化研究  

Study on dynamic changes of acetylcholine esterase(AChE) after middle cerebral artery occlusion with light and electron microscope

作　　者：谭斐[1] 金武[1] 牛世贵[1] 

机构地区：[1]中国医科大学第二临床学院神经内科,辽宁沈阳110003

出　　处：《中风与神经疾病杂志》2001年第1期16-18,共3页Journal of Apoplexy and Nervous Diseases

基　　金：辽宁省科委社发基金资助项目!(992250010)

摘　　要：目的 探讨脑梗死后胆碱能神经系统的变化特点。方法 利用血管内栓线法制备大鼠右侧大脑中动脉栓塞（MCAO）模型，于MCAO后0.5h、1h、2h、3h、6h分别进行光镜乙酰胆碱酯酶（AChE）染色及病理染色，并进行电镜AChE活性的观察。结果 光镜下MCAO 后1h AChE活性开始下降，以第IV细胞层染色优势消失为特点，自2h随梗死时间延长，酶活性进一步下降。电镜下正常组织AChE酶颗粒主要分布在突触前后膜及突起中，半暗区AChE酶颗粒数量及密度降低，梗死区酶颗粒几乎消失。结论 急性脑梗死后AChE活性变化出现在形态学改变之前，且与细胞的损伤密切相关，脑梗死后较早即发生了胆碱能神经系统的异常。Objective To study the dynamic changes of cholinergic system after cerebral infarction. Methods A rat model of right middle cerebral artery occlusion(MCAO) was made by insertion of an intraluminal suture. The enzyme histochemistry study of Acetylcholine esterase (AChE) was done at 0.5h、1h、2h、3h、6h respectively after MCAO under light microscope. The activity changes of AChE under electron microscope were also done. Results Under light microscope the activity of AChE began to change at 1h after MCAO, showing th e weakening of staining priority in the fourth cell layer, and continued to decrease with MCAO time extending from 2h after MCAO. Under electron microscope, the AChE enzyme particles were located in the processes、the anterior and posterior membranes of the synapses which decreased both in quantity and density in the ischemic penumbra after MCAO, and almost disappeared in the infarcted core. Conclusion The activit y of AChE changes before morphologic changes appeared after MCAO.The cholinergic system become abnormality in the early stage after cerebral infarction.

关 键 词：乙酰胆碱酯酶 酶组织化学 大脑中动脉栓塞 光镜 电镜 大鼠 

分 类 号：R743.33[医药卫生—神经病学与精神病学]