MHC-Ⅱ类抗原在角膜碱烧伤后眼组织的动态改变  

MHC classⅡ positive cells in the eye tissue of rats after alkali burn of cornea

作　　者：赵敏[1] 陈家祺[2] 杨培增[2] 

机构地区：[1]重庆医科大学临床学院眼科,重庆400016 [2]广州中山医科大学中山眼科中心,广州510060

出　　处：《重庆医科大学学报》2001年第1期63-63,68,共2页Journal of Chongqing Medical University

基　　金：美国中华医学基金会资助项目!(93-594)

摘　　要：目的： 探讨角膜碱烧伤后角膜溃烂溶解穿孔以及眼内炎症的免疫学机制。方法：在大鼠角膜上制作碱烧伤模型，在烧伤后的不同阶段，制备角膜、虹膜、脉络膜以及视网膜铺片，采用卵白素-生物素过氧化物酶免疫组化方法（ABC法），检测眼组织MHC-Ⅱ类抗原阳性细胞。结果： 烧伤初期，角膜及虹膜即有MHC-Ⅱ类抗原阳性细胞的轻度增多；在角膜溶解穿孔阶段，MHC-Ⅱ类抗原阳性细胞大量出现，密集分布于角膜缘及溃疡溶解处，以及虹膜、脉络膜组织中，且在形态学上也发生了一些变化。当烧伤后期溃疡愈合角膜病变稳定时，虹膜上依然见较多阳性细胞。正常视网膜细胞未见MHC-Ⅱ类抗原的表达，但在烧伤后角膜溶解期以及稳定期，均可见Ⅱ类抗原阳性细胞。结论： 免疫反应可能参与了严重角膜碱烧伤角膜溶解及眼内炎症的病理过程，其发生机理可能与自身免疫反应有关。Objective: To investigate immunological mechanism of corneal melting, perforation and ophthalmitis after alkali burn. Methods: A model of severe corneal alkali burn was established in rats. Immunohistochemical method (ABC method) was carried out on wholemounts of the cornea, iris, choroid and retina at different time after corneal burns to detect MHC class Ⅱ positive cells. Results: In the early stage of the burn, slight increase of the number of MHC class Ⅱpositive cells in the cornea and iris was found. At the time of corneal melting and perforation, a massive influx of the positive cells was not only seen at the limbus but also in the corneal melting area, iris and choroid. Morphological changes of the cells were also noted. In restoration stage, a number of positive cells was also found in iris. In the normal control group and in the early stage of the burn, the MHC class Ⅱantigen was not expressed in the retina tissue, but at the the time of corneal melting and the restoration stage MHC class Ⅱ positive cells were discovered. Conclusion: The immunological reaction is involved in the pathogenesis of the eye tissue damage induced by severe alkali burn of cornea and the mechanism may be related to autoimmune reaction.

关 键 词：角膜碱烧伤 动物模型 NMH-Ⅱ类抗原 免疫学 免疫组织化学 铺片技术 

分 类 号：R779.130.2[医药卫生—眼科]