Detection of microRNA expressions in tissues and exfoliative cells reveals the potential role of miR-203 in oral lichen planus  

组织与脱落细胞中的microRNA表达量检测指示miR-203在口腔扁平苔藓中的潜在作用(英文)

作　　者：冯震东 史闻[1,2,3] 蔡志刚[3] 华红[3] 周德敏[1,2] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室 [2]药学院化学生物学系,北京100191 [3]北京大学口腔医学院,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》2014年第5期279-286,共8页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No.91029711)

摘　　要：Oral lichen planus （OLP） is a chronic inflammatory disorder and premalignantlesion, of which the mechanisms are still obscure. In the present study, the expression levels of miR-96/182/183 cluster, miR-203, miR-375, and miR-769-5p in both tissues and exfoliative cells of OLP patients as well as healthy volunteers were detected, differentially expressed miRNAs were identified and their correlation with OLP was evaluated by a biplot method. Experimental results show that miR-203 is significantly up-regulated in patient lesion tissues in comparison to volunteer mucosa tissues. Moreover, the contra- dictory insignificant expression changes of miR-203 as well as miR-96/182/183 cluster in comparisons of exfoliative cell samples suggest that different cell compositions in OLP lesion have distinct miRNA regulation, which accords with the histological heterogeneity of OLP. Finally, biplot analyses indicate the expression of miR-203 and miR-96/182/183 cluster are positively correlated in patient lesions. These results provide miR-203 as a molecular indicator of heterogeneity of OLP, and also a potential diagnostic biomarker or therapeutic target that deserves further studies.口腔扁平苔藓(OLP)是一种癌前病变和慢性炎症,其损伤具有组织学异质性,多种类型的细胞参与其发生与恶性转化,而其分子机制尚不明确。本研究检测了来自于OLP患者和健康志愿者口腔组织和脱落细胞中miR-96/182/183簇、miR-203、miR-375、miR-769-5p的表达水平,并通过双标图法评价了显著差异表达的miRNA与OLP的相关性。研究发现,相对于志愿者正常组织,miR-203在患者病损区组织中显著高表达,其变化程度明显高于其它miRNA。与此相反,miR-203及miR-96/182/183簇的表达在脱落细胞中则没有显著差异,提示OLP病损区中不同类型细胞对miRNA表达的调控不同,可能与OLP的组织学异质性相关。双标图分析表明miR-203与miR-96/182/183簇的表达量在患者病损区组织中呈正相关。本研究结果表明miR-203不仅在分子层次显示了OLP的组织学异质性,还可能成为潜在的诊断标志物和治疗药物靶标。

关 键 词：Oral lichen planus MICRORNA RT-QPCR Biplot method miR-203 

分 类 号：R781.5[医药卫生—口腔医学]