环胞霉素A对白血病细胞增殖的影响  

Study on the effect of cyclosporin A on the proliferation of leukemic cells

作　　者：沈蕾[1] 黄海[1] 汤静燕[2] 陈静[2] 王耀平[3] 朱亚忠[3] 

机构地区：[1]上海第二医科大学附属上海儿童医学中心 [2]上海市儿科医学研究所 [3]上海第二医科大学附属新华医院,200092

出　　处：《临床儿科杂志》2001年第1期37-39,共3页Journal of Clinical Pediatrics

基　　金：上海高校青年基金

摘　　要：为研究环胞霉素A(CsA)的抗白血病潜能,应用四甲基偶氮唑蓝法及细胞活力试验于体外观察CsA对各种不同来源白血病细胞株(包括T细胞性Jurkat、molt-4、CCRF-CEM、前B细胞性白血病细胞株Nalm-6及急性髓性白血病细胞株K562,耐药细胞株K562/AO2)的增殖及活力影响。结果显示临床耐受剂量CsA对所有细胞株包括多药耐药细胞株的活力和增殖均有明显的抑制作用。提示CsA有可能成为治疗白血病的一种安全有效的新药。In order to investigate the potential anti-leukemic effect of cyclosporin A (CsA), MTT method and cell viability assay in vitro were carried out in this study to observe the effect of CsA on the proliferation and cell viability of various leukemic cell lines, such as T-cell Jurkat, Molt-4, CCRF-CEM, Nalm-6, K562 and multi-drug-resistant leukemic cell line K562/AO2. The results fully showed that CsA did possess the same cytotoxic action on all the leukemic cell lines, particularly including multi-drug-resistant leukemic cell line,and could then inhibit the proliferation and cell viability of these leukemic cells, thereby indicating that CsA might be applied as one of the new, safe and effective anti-leukemic agents when used with clinically adoptable dosage in leukemias.

关 键 词：环胞霉素A 白血病 药物治疗 细胞增殖 

分 类 号：R733.7[医药卫生—肿瘤] R730.53[医药卫生—临床医学]