展神经损伤后神经核内神经再生的研究  

Study of the neural regeneration in the abducens nucleus after abducens nerve injury

作　　者：张毅[1] 周凌云[2] 虞昊[1] 

机构地区：[1]南通大学第二附属医院神经外科,江苏南通226001 [2]哈尔滨医科大学附属第一医院针灸科,黑龙江哈尔滨150001

出　　处：《海南医学》2014年第9期1249-1251,共3页Hainan Medical Journal

基　　金：黑龙江省自然科学基金项目(编号:ZD201211);南通市社会事业科技创新与示范计划项目(编号:HS2012031)

摘　　要：目的探讨展神经损伤后展神经核内神经再生的情况。方法选用健康雄性Beagle犬4只,经颞底入路显微手术,解剖、暴露展神经脑池段行展神经切断,并测定展神经功能。术后连续7 d静脉注射BrdU,术后28 d灌注取材,犬脑行冠状位冰冻切片。行nestin/BrdU、DCX/BrdU、GFAP/BrdU免疫荧光双标检测。结果 Beagle犬眼球呈内斜视,损伤侧展神经核内可见nestin/BrdU阳性神经前体细胞,DCX/BrdU阳性新生神经元前体细胞以及GFAP/BrdU阳性星形胶质细胞。对侧展神经核内未见相关阳性细胞。结论展神经损伤后展神经核内出现神经再生现象。Objective To explore the neural regeneration in the abducens nucleus after abducens nerve in-jury. Methods Four healthy male Beagle dogs were selected and subtemporal approach was adopted. By using mi-crosurgery to expose the cisternal segment of the abducens nerve, the abducens nerve was cut and function test was measured. Dogs were injected with BrdU at 1 to 7 days post-injury to allow identification of differentiated cells and sacrificed at 28 days for immunofluorescence of nestin/BrdU, DCX/BrdU, GFAP/BrdU. Results Ocular motility dis-orders caused by abducens nerves injury emerged in the injured eye of Beagle dogs. Nestin/BrdU double positive neu-ral progenitor cells, DCX/BrdU double positive newborn neuronal progenitor cells and GFAP/BrdU double positive newborn astrocytes were found in the abducens nucleus after abducens nerve injury. Conclusion Neural regenera-tion occurred in the abducens nucleus of Beagle dogs with abducens nerve injury.

关 键 词：展神经损伤 展神经核 神经再生 

分 类 号：R-332[医药卫生]