经皮瘤内注射艾迪注射液对兔移植性VX2肝肿瘤VEGF表达的影响  

Expression of VEGF in the implanted VX2 liver tumor cells in rabbits with percutaneous intratumor AiDi injection

作　　者：陈翼 王玎玲 何重阳 

机构地区：[1]重庆市红十字会医院江北区人民医院放射科,重庆400020

出　　处：《医学影像学杂志》2014年第5期859-864,共6页Journal of Medical Imaging

摘　　要：目的探讨超声引导下复方中药艾迪注射液瘤内注射治疗兔移植性VX2肝肿瘤的作用效果及机制。方法建立新西兰大白兔肝VX2移植性肿瘤模型28只,随机分为3组,即艾迪注射液局部注射治疗组(12只)、经皮无水乙醇注射(percutaneous ethanol injection,PEI)组(8只)、生理盐水局部注射治疗组(8只)。全部动物在接种2周后每隔3天向肿瘤内注入治疗药物,4次后处死。于术前术后兔耳缘静脉抽血行血生化检查,同时行64排螺旋CT扫描测量并计算肿瘤体积、肿瘤增长率及肿瘤坏死率,以及进行免疫学、组织细胞学与细胞超微结构的观察。结果各实验组病灶均进行性增大,但艾迪注射液治疗组肿瘤增长率及坏死率最小,与生理盐水组比较差异均有统计学意义(P<0.05),与无水乙醇治疗组比较差异无统计学意义;艾迪注射液治疗组血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达水平最低,低于无水乙醇治疗组和生理盐水组,有显著差异性(P<0.05);同时,艾迪注射液治疗组有1例出现肝内转移灶,而无水乙醇及生理盐水治疗组分别有4例和5例出现转移灶,有显著差异性(P<0.05);艾迪注射液治疗组AST、ALT、ALP、BUN及白细胞计数治疗前后均无明显差异,组内及组间比较无统计学意义(P>0.05)。结论艾迪注射液能明显抑制兔肝移植性VX2肿瘤VEGF蛋白的表达。Objective To explore the principle ,effect and method of percutaneous AiDi injection for tumor ablation . Methods Twenty-eight new zealand white rabbits with implanted liver VX2 tumors were randomly divided into three groups：AiDi injection group （n=12） ,percutaneous ethanol injection （PEI） group （n=8） ,and physiological saline group （n=8） .Three weeks after transplantation ,the drugs were injected into tumors of all the rabbits once every 3 days .After 4 times injection ,the rabbits were killed .The tumors were measured 3 dimensionally with CT ,while the rates of tumor necrosis ,the volume of tumor and the growth rate of the tumor were calculated .Liver and kidney function tests （AST , ALT ,ALP and BUN） as well as white blood cell counts were assessed before and after treatment ,and CT examination was performed at the same time .The morphological and the ultramicrostructural changes were also investigated under light mi-croscope or electron microscope .Vascular endothelial growth factor （VEGF） and expression were examined by immuno-histochemistry .Results Tumors in all groups kept growing after the procedure .Among all 3 groups ,tumor growing rate for AiDi injection group was the lowest and the necrotic rate was the highest （ P 〈0 .05） ,while no significant difference was found between AiDi injection group and PEI group （ P 〉 0 .05） .There was no evident change of liver and kidney function before and after treatment in the 3 groups （ P〉0 .05） .The expression of VEGF protein was statistically lowest of the AiDi injection group （ P〈0 .05） .The expression of VEGF protein in the PEI group was lower than that of the sa-line group .Conclusion AiDi injection can inhibite the expression of VEGF of in the liver VX 2 tumors tissue .

关 键 词：兔 肝脏 VX2 模型  动物 艾迪注射液 

分 类 号：R735.7[医药卫生—肿瘤] R332[医药卫生—临床医学]