激光散斑成像技术在大鼠大脑中动脉栓塞模型侧枝循环观测中的应用  

Laser Speckle Contrast Imaging of Collateral Blood Flow during Cerebral Infarction in Rats

作　　者：闵喆[1] 李昌盛[2] 方瑜[1] 邱占东[1] 张苏明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]湖北医药学院附属太和医院急诊科,湖北十堰442000

出　　处：《神经损伤与功能重建》2014年第3期191-194,共4页Neural Injury and Functional Reconstruction

摘　　要：目的:利用激光散斑成像(LSI)技术观察大鼠大脑中动脉栓塞(MCAO)模型侧枝循环开放情况及局部脑血流的变化。方法:成年雄性SD大鼠9只,使用改良线栓法制备永久性大脑中动脉栓塞(pMCAO)模型。利用LSI技术观测术后90 min内缺血半球皮质血流变化及侧枝循环开放情况,术后24 h TTC染色法观察脑梗死体积。结果:造模成功后大鼠右侧大脑半球皮质大脑中动脉供血区血流明显减少,部分大鼠随着侧枝循环逐渐开放局部血流不同程度恢复,另一部分大鼠血流恢复缓慢。结论:LSI技术时间空间分辨率高,成像结果直观。ObjectiveTo study the collateral blood flow and focal cerebral blood flow （CBF） in middle cerebral artery occlusion （MCAO） rats by laser speckle imaging （LSI）. Methods： A permanent MCAO （pMCAO） model was generated in male SD rats （n=9） by the intraluminal filament technique. LSI was used to measure CBF and collateral blood flow in the ischemic cortical area and MCA territory after 90 min. The brain was stained with TTC to determine the infarct size 24 h later. Results： CBF was decreased in both the hemispheric cortical area and MCA territory in the first 15 min and increased blood flow velocity was observed in some animals later. Conclusion： LSI has high spatial and temporal resolution, and offers visual imaging.

关 键 词：大鼠 大脑中动脉栓塞 激光散斑成像 侧枝循环 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]