逍遥散对肝郁型拟阿尔茨海默病小鼠的影响  

Effects of Xiaoyaosan on Mice Model of Alzheimer's Disease with Stagnation of Liver-QI

作　　者：吴磊彬 邱昕[1] 陈国华[1] 魏丹[1] 邵卫[1] 李俐娟[1] 张忠文[1] 杨洁[1] 吴潇[1] 蔡薇娜 周晓香[1] 熊晶[1] 

机构地区：[1]武汉市中西医结合医院神经内科,武汉430022

出　　处：《神经损伤与功能重建》2014年第3期203-204,245,共3页Neural Injury and Functional Reconstruction

基　　金：湖北省卫生厅青年科技人才基金(No.QJX2010-44);湖北省自然科学基金(No.2011CDB298)

摘　　要：目的:观察逍遥散对慢性应激下拟阿尔茨海默病(AD)小鼠记忆能力和血清单胺类神经递质的影响。方法:将50只小鼠随机分为空白对照组﹑D-半乳糖组﹑复合模型组﹑逍遥散组﹑艾司西酞普兰组,每组10只。空白对照组给予生理盐水皮下注射,D-半乳糖组﹑复合模型组﹑逍遥散组﹑艾司西酞普兰组均皮下注射D-半乳糖溶液42 d造模。造模第29天开始,逍遥散组给予逍遥散灌胃,艾司西酞普兰组给予艾司西酞普兰灌胃,连续14 d;每日灌胃完成后,复合模型组、逍遥散组和艾司西酞普兰组均给予慢性束缚应激刺激,每天6 h,连续14 d。实验结束后,对各组进行避暗试验,并检测血清5-羟色胺(5-HT)和去甲肾上腺素(NE)含量。结果:避暗试验结果显示,与空白对照组和D-半乳糖组比较,复合模型组潜伏期缩短,错误次数增多;与复合模型组比较,逍遥散组和艾司西酞普兰组潜伏期延长,错误次数减少,逍遥散组和艾司西酞普兰组相比无明显差异。与空白对照组和D-半乳糖组比较,复合模型组5-HT和NE均明显降低;与复合模型组比较,逍遥散组和艾司西酞普兰组5-HT和NE均有升高,逍遥散组较艾司西酞普兰组升高更明显。结论:慢性束缚应激刺激联合D-半乳糖注射可成功建立肝郁型AD病证结合模型;逍遥散能明显改善此模型的记忆能力,且提升血清5-HT和NE的含量。ObjectiveTo observe effects of Xiaoyaosan on the memory ability and neurotransmitters expression in Alzheimer＇s disease （AD） mouce model with chronic mild stress. Methods： Fifty mice were randomly divided into five groups： blank group, D-Gal group, compound group, Xiaoyaosan group and Escitalopram group, with 10 mice in each group. The blank group was injected with normal saline, and the others were injected with D-gal for 42 days. From the twenty-ninth day, the mice in the Xiaoyaosan group were intragastrically administrated with Xiaoyaosan, and in the Escitalopram group with escitalopram. After the intragastric administration, the mice in both the Xiaoyaosan and Escitalopram groups were subjected to chronic stress for 6 hours each day, lasting for 14 days. Then Dark evading test and NE and 5-HT levels in all the mice were evaluated. Results： Compared to the groups of blank and D-gal, the escape latency time in the compound group was shortened and error times were increased in the Dark evading test, while the 5-HT and NA levels were decreased markedly. Compared to the compound group, the escape latency time in the Xiaoyaosan and Escitalopram groups were prolonged and error times were reduced, while 5-HT and NA were increased. Conclusion： Chronic mild stress and D-gal combination can be used to establish AD mouce model with stagnation of liver-QI. Xiaoyaosan can improve memory function and increase the serum 5-HT and NE levels in AD mice.

关 键 词：慢性束缚应激刺激 逍遥散 阿尔茨海默病 神经递质 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.05[医药卫生—临床医学]