聚桂醇对体外培养人脐静脉内皮细胞的促凝血活性的影响  

Effect of lauromacrogol on procoagulant activity of human umbilical vein endothelial cells

作　　者：刘俊[1] 胡腾龙[1,2] 刘明朋[3] 李席[1] 于洋[1] 顾玉州 

机构地区：[1]哈尔滨医科大学附属第一医院口腔颌面外科,150001 [2]哈尔滨医科大学附属口腔医院口腔解剖生理教研室 [3]山东省潍坊市中医院口腔颌面外科

出　　处：《现代口腔医学杂志》2014年第3期146-149,共4页Journal of Modern Stomatology

基　　金：哈尔滨医科大学附属第一医院院基金(2012YB010)

摘　　要：目的观察聚桂醇对体外培养人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)表面磷脂酰丝氨酸(phosphatidylserine,PS)暴露及其促凝血活性的影响。方法不同浓度的聚桂醇(0,10,20,30,40,50μg/ml)处理HUVECs 24小时。通过检测凝血时间分析相关促凝血活性。乳黏素(lactadherin)作为检测HUVECs的PS暴露的探针和凝血抑制剂。通过流式细胞仪和荧光倒置显微镜检测和观察HUVECs的PS暴露。结果凝血时间随着聚桂醇浓度的增加而显著缩短,并且具有浓度依赖性,药物处理组与未处理组间差异有统计学意义(P<0.05)。在抑制实验中,乳黏素起到明显的抑制作用,而抗组织因子抗体(Anti-TF)没有明显的抑制效果。在流式细胞仪和荧光倒置显微镜的检测和观察下,清晰地呈现出HUVECs的PS暴露。结论 HUVECs的PS暴露增多与其促凝血活性的增强具有聚桂醇浓度依赖性,聚桂醇通过增加HUVECs表面的PS暴露增强其促凝血活性。Objective To observe the effect of lauromacrogol on phosphatidylserine （PS） exposure and relevant procoagulant activity （PCA） of human umbilical vein endothelial ceils （HUVECs）. Methods HUVECs were treated with lauromacrogol of different concentrations （0, 10, 20, 30, 40, 50 μg/ml） for 24 hours. PCA of the HUVECs was evaluated using clotting time. Lactadherin was used as a probe for detecting the PS exposure and inhibiting coagulation. PS exposure of HUVECs was detected by flow eytometry and fluorescence microscope. Results With the concentration of lauromacrogol increasing, coagulation time was significantly shortened. Between drug-treated group and the untreated group were statistically significant differences （P〈0.05）. The PCA of HUVECs was inhibited effectively by lactadherin through blockade of PS. Anti-TF showed no obvious inhibiting effect. PS exposure of HUVECs was clearly presented by flow cytometry and fluorescence microscope. Conclusion Lauromacrogol dose-dependently increased the PS exposure and consequent PCA of HUVECs. Lauromacrogol enhanced procoagulant activity with increased PS exposure of HUVECs.

关 键 词：聚桂醇 人脐静脉内皮细胞 磷脂酰丝氨酸 乳黏素 

分 类 号：R739.8[医药卫生—肿瘤]