富血小板血浆复合物促进牙周骨缺损修复的实验研究  

The effect of platelet-rich plasma composites on the regeneration of rats periodontal tissue

作　　者：高云飞[1] 郭良微[1] 周晋[2] 李思敏[1] 

机构地区：[1]哈尔滨医科大学附属口腔医院牙周病科,150001 [2]哈尔滨医科大学附属第一医院血液科

出　　处：《现代口腔医学杂志》2014年第3期155-158,共4页Journal of Modern Stomatology

基　　金：黑龙江省博士后基金资助(LBH-Z11065);黑龙江省自然科学基金项目(D200831)

摘　　要：目的探讨富血小板血浆-褪黑素-骨水泥复合物对大鼠牙周骨缺损修复的作用。方法人工构建40只雄性SD大鼠下颌双侧第三磨牙颊侧牙周骨缺损模型(共80个);随机分为5组,在缺损处分别填入富血小板血浆-磷酸钙骨水泥(PRP-CPC)、褪黑素-磷酸钙骨水泥(MT-CPC)、富血小板血浆-褪黑素-磷酸钙骨水泥(PRP-MT-CPC)、磷酸钙骨水泥(CPC),空白对照组不加任何材料。术后观察其一般情况并于14、21天处死动物,取组织标本,作X线片检查及组织学切片,观察各组骨组织愈合情况。结果术后14天和21天各组骨缺损区均有新骨形成,成骨量随时间的推移而增加。经影像学检查和组织学评估,各组的成骨能力依次为:PRP-MT-CPC组>PRP-CPC组/MT-CPC组>CPC组>空白对照组,有统计学差异(P<0.05),PRP-CPC组与MT-CPC组差别无显著性意义(P>0.05),其中PRPMT-CPC组成骨能力最强。结论 PRP-MT-CPC复合物能够有效促进大鼠牙周骨缺损的修复。Objective To investigate the effect of platelet- rich plasma （PRP）-melatonin （MT）-calcium phosphate cement （CPC） composites on the regeneration of rats＇ periodontal tissue. Methods Mandibular first molars of male SD rats were divided randomly into five groups, with eighteen teeth in each group. Periodontal fenestration defects were created at the buccal side of the molar roots by surgery. PRP-MT-CPC, PRP-CPC, MT-CPC and CPC were applied to the defect,or the defect was left untreated. After 14, 21 days of implantation,the radiological and histological observations were performed to investigate the bone healing of the defect. Results All of the defects treated with transplant materials exhibited new bone formation 14 and 21 days postoperatively,increasing with time.The radiological evaluation showed that the ability of new bone formation was arranged in 5 groups as follows：PRP-MT-CPC group〉PRP- CPC group/MT-CPC 〉CPC group 〉control group, the difference was significant between them （P〈0.05）. Successful bone regeneration was observed at the bone defect applied（treated） with PRP-MT-CPC, which is marked contrast to other groups. Conclusion The application of PRP-MT-CPC composite is the effective way to facilitate the regeneration of periodontal fenestration defects.

关 键 词：富血小板血浆 褪黑素 磷酸钙骨水泥 牙周骨缺损 

分 类 号：R781.42[医药卫生—口腔医学]