NT-3基因修饰OECs-NSCs-复合细胞支架自体移植治疗脑出血的实验研究  

Treatment of autotransplantation of NT-3 gene modified OECs and NSCs complex adhering to scaffold on spontaneous cerebral hemorrhage

作　　者：汪春娟[1] 杨锦珊[1] 林华伟 付佩彩[1] 郭守刚[3] 唐荣华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]山东医学高等专科学校 [3]山东大学附属省立医院神经内科,济南250021

出　　处：《中华医学杂志》2014年第19期1483-1487,共5页National Medical Journal of China

基　　金：国家自然科学基金（81171171,30970991）;山东省自然科学基金（ZR2009CQ028,Y2007C043）

摘　　要：目的 观察神经营养因子-3（NT-3）基因修饰嗅鞘细胞-神经干细胞（OECs-NSCs）复合细胞支架自体移植对脑出血大鼠运动功能的影响.方法 建立Wistar大鼠尾状核脑出血模型,完全随机化分为A组：NT-3基因修饰的OECs-NSCs-复合细胞支架移植组;B组：NT3-OECs-NSCs-复合细胞移植组;C组：支架移植组;D组：空白对照组.各组移植后第1、3、7、14和30天进行运动神经功能评分,并经免疫组织化学染色及荧光染色鉴定移植细胞在大鼠脑内的生存、分布及分化情况.结果 A、B组在移植后第7、14、30天神经功能评分分别为（2.12 ±0.12、1.50±0.11、0.52±0.08）、（2.10±0.16、1.79±0.09、0.91±0.10）明显低于C、D组;C组与D组间差异无统计学意义（P＞0.05）.在第14天和第30天,A组评分明显低于B组（P ＜0.05,P＜0.01）.免疫组织化学方法证实A组移植后存活的NSCs数量较多、向损伤区迁徙的细胞较多.结论 NT-3基因修饰OECs-NSCS-复合细胞支架移植治疗能显著改善脑出血大鼠的运动功能,且移植的NSCs具有较强的存活、迁移及分化能力.Objective To explore the effects of autotransplantation of NT-3 gene modified olfactory ensheathing cell （OEC） and neural stem cell （NSC） complex adhering to collagen protein-heparin sulfate scaffold on motor function of rats with cerebral hemorrhage.Methods The cerebral hemorrhage model was established with caudate nucleus bleeding in Wistar rats.The animals were randomly divided into 4 groups：A （transplantation of NT-3 modified OEC-NSC complex adhering to scaffold）,B （transplantation of NT-3 modified OEC-NSC complex）,C （transplantation of scaffold） and D （blank control group）.The motor function of hind limbs was assessed at Day 1,3,7,14 and 30 post-transplantation respectively.The survival,distribution and differentiation of transplanted cells were tested by immunohistochemistry and fluorescent staining.Results The neurological functional score of group A （2.12 ± 0.12,1.50 ± 0.11,0.52 ±0.08）or B（2.10 ±0.16,1.79 ±0.09,0.91 ±0.10）was obviously inferior to group C/D at Days 7,14 and 30.No significant difference existed between groups C and D （P 〉 0.05）.The scores of A were markedly lower than those of B at Days 14 and 30 （P 〈 0.05,P 〈 0.01）.The numbers of surviving NSCs and cells migrating to focal area after transplantation was much more in group A than those in other groups.Conclusion Autotransplantation of NT-3 gene modified OEC-NSC complex adhering to collagen proteinheparin sulfate scaffold may markedly ameliorate the motor function of cerebral hemorrhagic in rats.And the transplanted NSCs have better capacities of survival,migration and differentiation.

关 键 词：嗅鞘细胞 神经干细胞 脑出血 神经营养因子-3 

分 类 号：R743.34[医药卫生—神经病学与精神病学]