Pinx1、hTERT mRNA在三种皮肤肿瘤组织中的表达及意义  

Expressions of PinX1 and hTERT mRNAs in three skin tumors and their significance

作　　者：陈前明[1] 方杰[2] 瓦庆彪[1] 袁伟[2] 吴波[1] 蔡琦[1] 路永红[1] 周培媚[1] 蒋存火[1] 

机构地区：[1]成都市第二人民医院皮肤科,610017 [2]遵义医学院附属医院皮肤科

出　　处：《中华皮肤科杂志》2014年第6期421-423,共3页Chinese Journal of Dermatology

基　　金：四川省科技支撑计划项目（20LOSZ0015）

摘　　要：目的探讨内源性端粒酶抑制基因PinX1及端粒酶逆转录酶hTERT在基底细胞癌、鳞状细胞癌、日光性角化病组织中的表达及其意义。方法实时定量PCR检测实验组36例3种皮肤肿瘤组织中内源性端粒酶抑制基因PinX1及端粒酶逆转录酶hTERT的mRNA表达水平，并以15例正常人皮肤组织作为对照组。结果实验组PinX1基因的mRNA表达水平（2．53±1．57）低于对照组（4．25±2．02），差异有统计学意义（t=3．273，P〈0．05）；hTERT基因的mRNA表达水平（6．37±3．81）明显高于对照组（1．62±0．99），差异有统计学意义（t=6．927，P〈0．05）；Pinx1的表达水平与hTERT表达的相关性无统计学意义（P〉0．05）。结论PinX1的下调及hTERT上调可能与3种皮肤肿瘤形成过程中端粒酶激活及维持机制有关。Objective To investigate the mRNA expressions of PinXl and hTERT in lesions of three skin tumors （including basal cell carcinoma （BCC）, squamons cell carcinoma （SCC） and actinic keratosis） as well as their significance, Methods This study included 36 tissue specimens from the lesions of 13 patients with BCC, 11 patients with SCC and 12 patients with actinic keratosis, as well as 15 tissue specimens from the normal skin of 15 individuals receiving surgical therapy. Real-time PCR was performed to quantify the mRNA expressions of PinX1 and hTERT in these specimens. Results The specimens from the lesions of the skin tumors showed a significantly lower expression of PinX1 mRNA （2.53 ± 1.57 vs. 4.25± 2.02, t = 3.273, P 〈 0.05）, but a higher expression of hTERT mRNA （6.37 ±3.81 vs. 1.62 ± 0.99, t = 6.927, P 〈 0.05） compared with those from the normal skin. There was no significant correlation between the mRNA expressions of PinX1 and hTERT （P 〉 0.05）. Conclusion The down-regulated PinX1 expression and up-regulated hTERT expression may be associated with the activation and maintenance of telomerase in the formation of the three skin tumors.

关 键 词：皮肤肿瘤 基因 Pinxl 基因 hTERT 

分 类 号：R739.5[医药卫生—肿瘤]