HPV分型检测在宫颈细胞学阴性患者宫颈病变诊断中的意义  

Significance of HPV-DNA genotyping in patients with negative cytology findings

作　　者：朱利[1,2] 汤伟伟[1,2] 叶宇齐[1,2] 邢玉霞[1,2] 万贵平[1,2] 

机构地区：[1]中国中医科学院江苏分院 [2]江苏省中西医结合医院妇产科,江苏省210028

出　　处：《江苏医药》2014年第10期1140-1141,1144,共3页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK20131457);中国中医科学院江苏分院院级课题(JSBN1304);(JSBY1309)

摘　　要：目的探讨高危型人乳头瘤病毒(HPV)分型检测在宫颈细胞学阴性患者宫颈病变诊断中的意义。方法对宫颈细胞学阴性的1000例患者行HPV DNA检测,HPV阳性的218例患者行高危型HPV分型检测,阴道镜下宫颈活组织检查。结果就诊人群中宫颈细胞学阴性,HPV感染阳性率为21.80%(218/1000)。218例病例中,病理证实宫颈上皮内瘤变(CIN)57例(26.15%)。其中,CIN1检出39例(17.89%),CIN2及以上检出18例(8.26%)。HPV16、18型在CIN2及以上病变者中感染率为6.88%,高于其他12种高危型者HPV阳性率1.38%(P<0.05)。结论高危型HPV分型检测在宫颈细胞学阴性患者宫颈病变的诊断中有重要价值;对HPV16、18型阳性患者,需行阴道镜检查。Objective To evaluate the value of detecting high-risk human papilloma virus （HPV） subtypes in the patients with negative cytology findings. Methods The HPV DNA was examined in 1000 patients with negative cytology findings, of whom 218 cases were high-risk HPV DNA positive. The colposcopy and cervical biopsy were performed in 218 cases and HPV genotyping test was performed. Results HPV infection rate in the 1000 patients with negative cytology findings was 21.80%. Of 218 patients with negative cytology findings and positive high-risk HPV, 57 （26.15%） cases were histologically confirmed to be with cervical intraepithelial neoplasia（CIN）, Of whom, 39 （17.89%） cases were histologically diagnosed as CIN1 and 18 （8.26%） cases were diagnosed as CIN2 and higher levels. The prevalence of CIN2 and higher levels of HPV16 and HPV18 positive groups was 6. 88%,which was significantly higher than 1.38% of the rest 12 kinds of highrisk HPV（P〈0.05）. Conclusion High-risk HPV subtype testing has an important diagnostic value for cervical lesions with negative cytology findings. Colposcopy should be considered in the patients with HPV16 and HPV18 positive.

关 键 词：人乳头瘤病毒 宫颈上皮内瘤变 

分 类 号：R737[医药卫生—肿瘤]