冷暴露小鼠尾动脉舒缩功能变化以及血管活性药物的作用特征  

The effect of hypothermia on the vasoconstriction and vasodilatation and concerned with vasoactive drugs

作　　者：韩文志[1] 宁萌[1] 黄景慧[1] 刘卫[1] 张雁芳[1] 崔文玉[1] 汪海[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所心血管药物研究中心,北京100850

出　　处：《中国应用生理学杂志》2014年第3期204-207,共4页Chinese Journal of Applied Physiology

基　　金：军队特需药品专项(2011ZXJ09105-03B);国家自然科学基金资助项目(81171870)

摘　　要：目的:研究不同温度条件下血管舒缩功能变化及哌唑嗪、山莨菪碱扩张血管作用变化特征,评价Vit E在低温条件下的内皮保护作用,探讨上述药物在冻伤预防过程中的应用前景。方法:利用血管条技术,观察小鼠尾动脉血管在8℃、16℃、25℃、37℃四个温度条件下的收缩及舒张反应特点,比较哌唑嗪、山莨菪碱在不同温度条件下扩血管作用差异。在冷暴露处理的同时预敷Vit E,观察其对低温条件下血管内皮依赖性舒张功能的改善作用。结果:①不同温度条件下苯肾上腺素诱发的血管收缩反应存在明显差异,温度越低,收缩幅度越小;②硝普钠浓度依赖的扩血管作用随着温度的降低明显增强;③与硝普钠作用特点类似,哌唑嗪、山莨菪碱在低温条件下的扩血管作用强于37℃组;④低温能够降低乙酰胆碱内皮依赖的扩血管作用,Vit E能够剂量依赖地对抗低温的影响。结论:随着温度的降低,苯肾上腺素作用下的血管收缩明显减弱,平滑肌靶点扩血管药物的作用显著增强。乙酰胆碱内皮依赖的扩血管作用随温度的下降有所降低,Vit E能够在一定程度上减小低温对乙酰胆碱扩血管作用的影响。Objective： To investigate the changes of vasoconstriction and vasodilatation under different temperature conditions and the protective effects of Vitamin E（Vit E） against endothelial injury induced by hypothermia. Methods： The tail arterial rings were prepared for isometric tension recording using multi wire myograph system. The effect of temperature on relaxation and construction was evaluated. Incubate the arterial rings with different concentration of Vit E when they were exposed to hypothennia, then acetylcholine（ACh）-induced endothelium-de- pendent relaxation was investigated to evaluate the activity of endothelial. Results： （1）The hypothennia could enhanced the dose-dependent construction induced by PE in mice tail artery. （2）Exposure to hypothermia also resulted in increase of sodium nitroprusside（SNP）-induced re- laxation. （3）Compared with the normal group, prasozin（PZ） and anisodamine（Ani） were more effective in relax tail arterial rings at 8℃. （4） After incubation with Vit E, the vascular relaxation responses to ACh increased in an endothelium-dependent manner, when compared with the hypothennia-treated group. Conclusion： The vascular function of constriction was attenuated by hypothennia, while the relaxation was increased. Vit E could prevent the hypothennia-induced decrease in vascular endothelial cells.

关 键 词：冷暴露 尾动脉 血管舒缩 哌唑嗪 山莨菪碱 VIT E 

分 类 号：R331[医药卫生—人体生理学]