一个新的人类肝癌相关基因的克隆和表达  

Cloning and expression of a human liver cancer related gene

作　　者：王征旭[1] 王红阳[2] 吴孟超[2] 

机构地区：[1]兰州军区兰州总医院普外科,730050 [2]第二军医大学东方肝胆外科医院,上海200438

出　　处：《中华外科杂志》2001年第3期185-187,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金! (3 0 0 0 0 0 77;3 982 5 114 );中国博士后科学资助金! (中博基 1999[10 ] );上海市博士后科学资助金资助项目

摘　　要：目的　研究和克隆新的肝癌相关基因 ,探索肝癌发生的分子机制。　方法　采用mRNA差异显示技术 ,分析原发性肝癌和癌旁肝组织基因差异表达情况 ,获得差异表达基因片断 ;通过筛选胎盘cDNA文库和基因组拼接 ,获得新基因全长cDNA ;通过Northern杂交的方法 ,分析所获得的新基因在 42对肝癌和对应癌旁肝组织中的差异表达 ,和在正常组织中的分布情况。　结果　克隆和鉴定了一个新的全长 170 6个核苷酸的肝癌相关基因STW 2 ,定位在染色体 18p11 2区带上 ;该基因在 42对肝癌组织中高表达的阳性率是 78 6 % (33/4 2 ) ,其阳性表达与肿瘤包膜的完整性、有无癌旁卫星灶相关 (P <0 0 5 ) ;该基因具有较为广泛的基因表达谱。　结论　克隆和鉴定了一个肝癌高表达新基因全长cDNA ,其高表达与肝癌细胞的浸润和转移能力有关。Objectives To study and clone aovel liver cancer related genes, and to explore the molecular basis of liver cancer genesis. Methods Using mRNA differential display polymerase chain reaction (DDPCR), we investigated the difference of mRNA in human hepatocellular carcinoma (HCC) and paired paracarcinoma tissue, and got a gene probe. By screening the placenta cDNA library and genomic homologous extend, we got a full-length cDNA named STW-2. We analyzed the expression of this novel gene in 42 pairs of human hepatocellular carcinoma, paracarcinoma and normal tissue by means of Northern Blot. Results We have got a full-length cDNA of liver cancer associated gene STW-2 submitted to GeneBank nucleotide sequence databases (Accession No. AF276707), which localized at chromosome 18p112. The positive expression rate of this gene was 786% (33/42) in hepatocellular carcinoma tissue, and the clinical pathological data showed that the STW-2 was closely related to the completion of tumor envelope and adjacant small satellite nodules lesions (P<005). The STW-2 was widely distributed in human normal tissue that was highly expressed in human lunge, brain tissue, and lowerly expressed in liver tissue. Conclusion A novel full-length cDNA differentially expressed was obtained in liver cancer, which was closely related to the invasive and metastatic tumor, and may be the later heredited change in HCC genesis.

关 键 词：肝细胞癌 基因表达 基因克隆 HCC CDNA MRNA差异显示技术 DDCR 

分 类 号：R735.7[医药卫生—肿瘤]