E1A因子在膀胱癌组织中的表达及其对基质金属蛋白酶-2的调控作用  

Expression of E1AF in bladder carcinoma and its regulation on MMP-2

作　　者：顾永鹏[1] 侯树坤[1] 杜志军[1] 闫征[2] 张小东[1] 王晓峰[1] 

机构地区：[1]北京大学人民医院泌尿外科,100044 [2]北京大学人民医院,中心实验室100044

出　　处：《中华外科杂志》2001年第3期226-228,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目! (3 9870 85 3 )

摘　　要：目的　测定人膀胱移行细胞癌组织中 ,不同病理分期的人细胞 5型腺病毒早期转录单位E1A因子 (E1AF)和基质金属蛋白酶 2 (MMP 2 )的表达 ,探讨二者之间关系及其临床意义。　方法　采用RT PCR和WesternBlot法 ,检测 41例人膀胱癌组织中E1AF和MMP 2的mRNA及蛋白表达 ,并运用计算机图像扫描分析仪测定图像的吸光度值 (A值 )进行半定量分析。　结果　 2 6例表浅性膀胱癌中 ,E1AF的阳性率为 2 6 90 % ,MMP 2和E1AF的A值分别为 36 0 9± 5 82和 2 9 0 8± 6 2 6 ;15例浸润性膀胱癌中 ,E1AF的阳性率为 6 6 6 7% ,MMP 2和E1AF的A值分别为 119 98± 10 44和 89 6 2±10 96。E1AF的阳性率、MMP 2和E1AF的A值在两组均有显著性差异 ;E1AF在G3级膀胱癌组织中的表达量显著高于G1级 ;MMP 2和E1AF的表达呈正相关 (r=0 5 36 ,P <0 0 1)。　结论　E1AF调节MMP 2的表达 ;Objectives To measure the expression of E1AF and matrix metalloproteinase (MMP-2) on tissues of bladder transitional cell carcinoma(BTCC) and to discuss their relationship and clinical significance. Methods 41 human BTCCs were studied by RT-PCR and Western Blot to determine the expression of mRNA and protein of E1AF and MMP-2 respectively. After that, computer-assisted image scan and half-quantitative analysis were performed. Results In 26 superficial BTCCs, the positive rate of E1AF was 2690%, and the OD value of MMP-2 and E1AF was 3609±582 and 2908±626 respectively. In 15 invasive BTCCs, the positive rate of E1AF was 6667%, and the OD value of MMP-2 and E1AF was 11998±1044 and 8962±1096 respectively. Significant differences in the OD values of E1AF and MMP-2 and positive rate of E1AF were observed between the two groups. The expression of E1AF in grade Ⅲ was higher than that in grade Ⅰ. The expression of E1AF was significantly associated with that of MMP-2 in the bladder tissues (r=0536,P<001). Conclusion The expression of MMP-2 is regulated by E1AF. The high expression of E1AF and MMP-2 is associated with the invasion and prognosis of BTCC.

关 键 词：膀胱肿瘤 基质金属蛋白酶类 癌基因蛋白质类 E1A因子 基因调控 MMP-2 

分 类 号：R737.14[医药卫生—肿瘤] R730.23[医药卫生—临床医学]