ING4基因表达上调对卵巢上皮性癌OVCAR细胞顺铂耐药性的影响  

Cisplatin resistance affected by ING4 cancer increases gene expression in epithelial ovarian cancer

作　　者：张芳 柳英兰[2] 

机构地区：[1]辽源矿业集团总医院泌尿外科,吉林辽源136201 [2]哈尔滨医科大学附属第一医院妇产科,黑龙江哈尔滨150001

出　　处：《现代肿瘤医学》2014年第6期1254-1258,共5页Journal of Modern Oncology

基　　金：2011年黑龙江省教育厅课题(编号:12521233)

摘　　要：目的:探讨ING4基因在表达上调的卵巢上皮性癌(卵巢癌)细胞系OVCAR细胞中对顺铂耐药性的影响。方法:脂质体介导的ING4-Ad及相应阴性对照片段转染人卵巢癌细胞OVCAR,实验分为3组:转染组、阴性对照组、空白对照组。实时荧光定量PCR技术检测3组细胞中ING4基因的表达;采用四甲基偶氮唑蓝(MTT)法测定各组细胞增殖抑制率和绘制细胞生长曲线,集落形成实验测定细胞生长能力,检测3组细胞对顺铂的敏感性,及不同浓度顺铂和作用不同时间后3组细胞生长的抑制率和凋亡率及细胞周期变化。结果:转染组、阴性对照组及空白对照组OVCAR细胞中ING4的表达量分别为66.76±11.40、1.28±0.09、1.22±0.08(P<0.01)。MTT比色法检测显示,转染组细胞生长速度减慢,对顺铂的敏感性下降,在相同条件下顺铂对转染组细胞生长的抑制率最低(P<0.01)。转染组细胞较阴性对照组生长缓慢,转染组细胞的集落形成率低于阴性对照组和空白对照组(P<0.05)。流式细胞仪检测显示,3组细胞的凋亡率随着顺铂浓度的升高和作用时间的延长而升高,呈剂量-时间依赖关系(P<0.05)。在相同条件下,转染组细胞的凋亡率、S期细胞比例分别低于阴性对照组和空白对照组(P<0.05)。结论:卵巢癌OVCAR细胞中ING4基因表达上调后,可抑制细胞增殖,并产生凋亡受阻,对顺铂的敏感性下降。Objective：To investigate the ING4 gene upregulation of epithelial ovarian cancer（ ovarian cancer ）on cisplatin -resistant cell lines OVCAR cells. Methods：The liposome -mediated ING4 -Ad and the corresponding negative control fragment was transfected into human ovarian cancer OVCAR,rats were divided into three groups：transfection group,negative control group and blank control group. Real-time PCR technology was used to detect the expression of ING4 gene. Using tetrazolium blue（ MTT）assay to make inhibition of cell proliferation and cell growth curve,colony formation assay measured the growth of the cells＇ ability. Results：In transfection group,negative control group and blank control group ING4 expression of OVCAR cells were 66. 76 ± 11. 40,1. 28 ± 0. 09,1. 22 ± 0. 08 （P〈0. 01）. MTT assay showed that the growth rate slowed transfected cells,decreased sensitivity to cisplatin,in transfected cell growth inhibition rate was the lowest（P〈0. 01）. Transfected cells grow slowly compared with the neg-ative control group,set transfected cell colony formation rate is lower than the negative control group and blank control group（P〈0. 05）. Flow cytometry showed that the three groups with the extension of the apoptosis rate increased con-centrations of cisplatin（P〈0. 05）. Conclusion：The ovarian OVCAR cells ING4 gene,inhibit cell proliferation,apop-tosis decreased sensitivity to cisplatin.

关 键 词：卵巢癌 生长抑制 顺铂 耐药 

分 类 号：R73-362[医药卫生—肿瘤] R737.31[医药卫生—临床医学]