兔髌下脂肪垫损伤模型中神经肽的变化  

CHANGES OF NEUROPEPTIDES IN RABBITS INFRAPATELLAR FAT PAD INJURY MODEL

作　　者：毕胜[1] 王福根[1] 侯京山[1] 陈素娟[1] 钟辉 李振甲[1] 

机构地区：[1]解放军总医院,康复医学科北京100853

出　　处：《中国疼痛医学杂志》2001年第1期33-35,共3页Chinese Journal of Pain Medicine

摘　　要：目的 :髌下脂肪垫损伤的病理基础是反复发作的慢性损伤以及脂肪垫夹挤和撞击引起脂肪垫增生、炎症。本研究通过建立膑下脂肪垫和滑膜损伤的模型 ,观察神经肽SP、CGRP和NPY的水平变化。方法 :应用水囊压迫兔膑下脂肪垫 ,水囊内压力保持 330～340mmHg ,配合膝关节屈伸运动 ,每天 2小时 ,压迫 7天后 ,于对照组与损伤组注入 0 .5ml生理盐水 ,抽取关节冲洗液 ,对观察指标进行放射免疫测定。结果 :SP :损伤组 71 .34± 2 4 .44pg/ml,对照组 2 1 .91± 1 4.44pg/ml,(P <0 .0 1 ) ;CGRP :损伤组 1 0 5.62± 1 7.38pg/ml,对照组 56.62± 2 0 .57pg/ml,(P <0 .0 1 ) ;NPY :损伤组 1 69.50± 95.2 1 pg/ml,对照组46.47± 36.41 pg/ml,(P <0 .0 5)。结论 :所检测的SPCGRPNPY等炎症介质损伤组与对照组相比在关节冲洗液中均明显升高 。Objective: The pathological basis of infrapatellar fat pad injury is repeated injury, and impingement of the infrapatellar fat pad causing fat pad proliferation and inflamation. The model of infrapatellar fat pad and synovium injury was established, and the level of SP, CGRP and NPY were observed. Methods: We used pockets of water compressing rabbit infrapatellar fat pad, the pressure in pockets of water remaining 330～340mmHg, accompanied by knee joint flexion and extension 2 hours every day. Seven days later, 0.9% NaCl 0.5ml was injected into the joint cavity in control and injury group. The joint flushing fluid was then extracted and measured by radioimmunoassay. Results: SP: injury group 71.34±24.44 pg/ml, control group 21.91±14.44 pg/ml, ( P <0.01) ; CGRP:injury group 105.62±17.38 pg/ml control group 56.62±20.57 pg/ml ( P <0.01); NPY:injury group 169.50±95.21 pg/ml control group 46.47±36.41 pg/ml ( P <0.05). Conclusion: SP, CGRP and NPY in injury group were significantly higher than in control group. The results demonstrate that neurogenic inflammatory mediators may play important role in the pathogenic mechanisms of infrapatellar fat pat injury.

关 键 词：髌下脂肪垫损伤 P物质 降钙素基因相关肽 神经肽Y 兔 

分 类 号：R363[医药卫生—病理学]