脑胶质瘤抑制因子Glob-N分子结构的初步鉴定  

PRELIMINARY STUDY OF THE MOLECULAR STRUCTURE OF GLOB-N:A GLIOMA INHIBITOR

作　　者：张健[1] 张宗城[1] 张积仁[1] 李鹏[1] 王兵[1] 林永成[2] 

机构地区：[1]第一军医大学珠江医院,广州510282 [2]中山大学化学系

出　　处：《解放军医学杂志》2001年第3期160-162,F003,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题! (编号 3 970 0 14 3 ;3 9870 813 )

摘　　要：为分析脑胶质瘤抑制因子N 红细胞糖苷脂 (Glob N)的分子结构 ,运用核磁共振仪及质谱仪分析了人O型血红细胞膜、牛脑及人脑Glob N的结构。结果显示 ,红细胞Glob N为四糖基神经酰胺 ,牛脑与人脑Glob N均为三糖基神经酰胺 ,3种Glob N中神经酰胺所含的碳原子数目不同 ,分别为 2 7、33和 2 6个。说明Glob N为一类中性鞘糖脂 ,其结构与组织来源相关。In order to study the molecular structure of Glob-N, a glioma inhibitor, Glob-N was separated from the membrane of human type O red blood cells, cow brain and normal human brain tissue respectively. Their structures were identified by using time-of-flight mass spectrometry, 1H-NMR and 13C-NMR. The results showed that the structure of Glob-N from the membrane of human type O red blood cells was proposed as globotetraosylceramide, while Glob-N from cow brain and normal human brain was globotriaoceramide, and the number of carbon atoms in the ceramide of the Glob-N was 27, 33 and 26 respectively. It suggested that Glob-N is a kind of neutral glycosphingolipids, and its molecular structure is different in different tissues.

关 键 词：中性糖苷神经鞘脂类 分子结构 肿瘤抑制因子 脑胶质瘤 Glob-N 

分 类 号：R739.41[医药卫生—肿瘤] R73－34[医药卫生—临床医学]