CMH为KBv_(200)耐药相关中性鞘糖脂  

CMH IS THE MULTIDRUG RESISTANCE(MDR) ASSOCIATED NEUTRAL GLYCOSPHINGOLIPID IN KBv_(200)CELL LINE

作　　者：张健[1] 汪森明[1] 赵燕[1] 张积仁[1] 

机构地区：[1]第一军医大学珠江医院,广州510282

出　　处：《解放军医学杂志》2001年第3期171-173,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 !(编号 3 9870 813 )

摘　　要：为了研究耐药细胞株KBv2 0 0 中性鞘糖脂表达的规律 ,采用改良的Hakomori法提取、纯化了KB及其耐药细胞株KBv2 0 0 的中性鞘糖脂 ,行高效薄层层析 ,分析其含量的异同 ;并以糖脂合成抑制剂PPMP抑制KBv2 0 0 中性鞘糖脂的合成 ,观察了其对KBv2 0 0 耐药性的逆转作用。结果发现 ,KBv2 0 0 的CMH和CDH表达较KB强 ,尤以CMH为著 ,PPMP能抑制KBv2 0 0 细胞CMH的合成 ,并能逆转KBv2 0 0 对长春新碱 (VCR)的耐药性。说明CMH为KBv2 0 0 耐药相关中性鞘糖脂 ,抑制耐药细胞CMH的合成可能是逆转肿瘤多药耐药的一种新方法。In order to study the relationship between the expression of neutral glycosphingolipids(N-GSLs) and MDR, N-GSLs in MDR cell line KBv 200 and its parental cell line KB were studied. The N-GSLs were extracted and purified from the KB and KBv 200 cells according to the modified method of Hakomori, then analysed by High Performance Thin Layer Chromatography (HPTLC). The effects of PPMP on the expression of N-GSLs in KBv 200 cell line were also studied by the above method, its reversion on the KBv 200 cells to Vincristine (VCR) was examined by MTT method. The results showed that the levels of CMH and CDH in KBv 200 cells were higher than in KB cells, CMH was much more markedly increased, PPMP could reverse MDR by inhibiting CMH synthesis. It suggested that CMH is MDR associated N-GSLs in KBv 200 cell line, and inhibition of CMH expression in tumor cells maybe a new way to reverse MDR.

关 键 词：多药耐药性 中性糖苷神经鞘脂类 PPMP CMH KBV200 肿瘤 

分 类 号：R73－34[医药卫生—肿瘤] R73－361[医药卫生—临床医学]