非小细胞肺癌MAGE-3基因的表达  

EXPRESSION OF MAGE-3 GENE IN NON-SMALL CELL LUNG CANCER

作　　者：黎涛[1] 孙耀昌[1] 徐志飞[1] 费月华[1] 

机构地区：[1]第二军医大学长征医院,上海200003

出　　处：《解放军医学杂志》2001年第3期201-203,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：为探讨以MAGE 3基因编码的肿瘤抗原作为靶点对非小细胞肺癌 (NSCLC)患者进行免疫治疗的可能性 ,采用逆转录 聚合酶链反应 (RT PCR)技术检测 3种人肺癌细胞系和 5 6例NSCLC标本及相邻正常肺组织标本MAGE 3mRNA的表达。结果显示 ,3种人肺癌细胞系均表达MAGE 3mRNA ;5 6例NSCLC标本中 ,30例表达MAGE 3mRNA ,其中鳞状细胞癌的表达率明显高于腺癌的表达率 (P <0 .0 1) ,而相邻正常肺组织均不表达MAGE 3mRNA。MAGE 3mRNA在NSCLC中呈高比例表达 ,提示该基因编码的肿瘤抗原可以作为对NSCLC患者进行免疫治疗的攻击靶点。To explore the possibility of using tumor antigen encoded by MAGE-3 gene as the target for immunotherapy for non-small cell lung cancer (NSCLC) patients, the expression of MAGE-3 mRNA in 3 human lung cancer cell lines and 56 NSCLC samples together with the adjacent normal lung samples was determined by RT-PCR. The results showed that all 3 human lung cancer cell lines expressed MAGE-3 mRNA; of the 56 NSCLC samples, 30 expressed MAGE-3 mRNA. The expression rate in squamous cell carcinoma was significantly higher than in adenocarcinoma, whereas none of the adjacent normal lung samples expressed MAGE-3 mRNA. This study suggests that MAGE-3 mRNA is expressed in a high percentage in NSCLC specimens, and its encoding tumor antigen may be the target for immunotherapy for NSCLC patients.

关 键 词：肺肿瘤 MAGE-3基因 免疫治疗 逆转录-聚合酶链反应 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]