relA基因在重组大肠杆菌BL21合成嘌呤核苷磷酸化酶作用研究  

Function of relA gene in production of PNPase in E.coli BL21

作　　者：段纪甫[1] 高黎荣[1] 李江[1] 付水林[1] 宫衡[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《工业微生物》2014年第3期30-34,共5页Industrial Microbiology

摘　　要：利用Red同源重组技术敲除大肠杆菌BL21中relA基因,获得△relA突变株。进一步研究表明在LB复合培养中relA基因对大肠杆菌的生长几乎没有明显影响,但是对其重组蛋白的合成有着较大的影响:降低了25%。结果表明,relA基因在大肠杆菌表达重组蛋白方面有着较重要的作用。The Red recombination system was used to knock out the relA gene in E. coli BL21 and then the ArelA mutant was obtained. Further study was conducted and results showed that the relA gene had no effect on its growth while significant difference in terms of synthesis of recombinant protein was found between these two strains. It reduced by 25 % compared with the original strain. Results showed that the relA gene may play an important role in synthesis of recombinant protein in E. coli BI21.

关 键 词：大肠杆菌 RED重组 relA基因 嘌呤核苷磷酸化酶 

分 类 号：Q78[生物学—分子生物学]