高产γ-氨基丁酸的谷氨酸棒杆菌工程菌的构建和发酵条件优化  

Construction of recombinant Corynebacterium glutamium for high production of γ-aminobutyric acid and its optimization of fermentation

作　　者：江君君 史锋[1,2] 王小元[1,2] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,无锡214122 [2]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122

出　　处：《工业微生物》2014年第3期53-58,共6页Industrial Microbiology

基　　金：江南大学食品科学与技术国家重点实验室自由探索资助课题(编号SKLF-TS-201103

摘　　要：为了实现γ-氨基丁酸(GABA)在微生物中"一步法"高效生产,本研究构建出一株高产GABA的谷氨酸棒杆茵工程菌ATCC 13032/pDXW10-gadB1-gadB2,可以直接将自身合成的L-谷氨酸转变成GABA,并对该工程茵的发酵培养基和发酵条件进行了初步优化。结果表明:培养7 h^8 h的种子液按发酵起始OD_(562)=1.6转接至发酵培养基(葡萄糖、玉米浆分别100 g/L、4 g/L),10 h添加PLP至O.1 mmol/L,发酵结束后胞外GABA可达(26.39±1.68)g/L。为工业化"一步法"生产GABA提供理论和实验基础。In order to establish a single-step de novo biosynthetic system for γ-aminobutyric acid （ GABA）, a recombinant C. glutamicum strain ATCC 13032/pDXWlO-gadBl-gadB2 was constructed, which could directly convert endogenous L- glutamate precursor into GABA. The fermentation medium and conditions were optimized preliminarily. The seed broth was cultivated for 7 h to 8 h and then inoculated into fermentation medium till the final optical density of 1.6. The glucose and corn steep liquid concentration in fermentation medium were 100 g/L and 4 g/L, respectively. During 6 h to 24 h, 2 g/L of urea was added for six times. At 10 h, 0.1 mmol/L PLP was added. After fermented for 84 h, the GABA production reached to （26.39 ± 1.68 ） g/L. This research provided a theoretical and practical basis for industrial single-step production of GABA.

关 键 词：Γ-氨基丁酸 谷氨酸棒杆菌工程菌 优化 

分 类 号：TQ921.4[轻工技术与工程—发酵工程]