拓扑异构酶Ⅱα,P糖蛋白在胆囊癌中的表达及其临床意义  

Expression of Topo Ⅱα and P-glycoprotein in gallbladder carcinoma and their clinical significance

作　　者：黄迅[1] 沈丹华[2] 陈祥柏[1] 虞有智[2] 许峰[1] 赵勇[1] 祝学光[1] 

机构地区：[1]北京大学人民医院普外科,100034 [2]北京大学人民医院病理科,100034

出　　处：《中华肝胆外科杂志》2001年第3期150-152,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的　从细胞分子水平对胆囊癌化疗的耐药性进行研究 ,为临床化疗药物的筛选提供理论依据并获得更有效的临床化疗效果。方法　应用免疫组织化学的方法 ,检测胆囊癌中的主要耐药基因拓扑异构酶Ⅱα(topoisomeraseⅡα ,TopoⅡα)、P糖蛋白 (P glycoprotein ,P gp)的表达情况 ,并结合病理组织学分级、临床分期进行研究。结果　TopoⅡα在胆囊癌中的表达 ( 91 7% )明显高于对照组 ( 10 % ) (P <0 0 5 )。P糖蛋白在胆囊癌中的表达 ( 5 8 3 % )高于对照组 ( 2 5 % ) (P <0 0 5 )。TopoⅡα在低分化癌的阳性率明显高于中高分化癌 ,但与肿瘤的TNM分期无关。P糖蛋白的表达与胆囊癌组织学分级、TNM分期均无关。结论　TopoⅡα在胆囊癌中明显高表达 ,选择TopoⅡα抑制剂进行化疗有助于提高化疗效果。Objective To investigate the expression of topoisomerase Ⅱα(Topo Ⅱα) and P-glycoprotein (P-gp) in gallbladder carcinoma.Methods The immunohisto-chemical staining for Topo Ⅱα and P-gp was performed in 36 patients with gallbladder neoplasm and 20 patients with chronic cholecystitis. The correlations between the expression of Topo Ⅱα and P-gp and clinical stage, pathological grade of gallbladder carcinoma were analyzed. Results 91 7% gallbladder carcinoma expressed Topo Ⅱα. The positive rate of Topo Ⅱα was higher in poorly differentiated carcinoma than in well differentiated one (P<0 05). 58 3% gallbladder carcinoma expressed P-gp and the expression was higher than in the control. Conclusions Topo Ⅱα was expressed highly in the gallbladder neoplasm and could be the target of anti-tumor drugs. Selection of the Topo Ⅱα inhibitors should be the key to improve the efficiency of chemotherapy.

关 键 词：胆囊肿瘤 DNA拓扑异构酶 ATP水解 P-糖蛋白 

分 类 号：R735.8[医药卫生—肿瘤]