MicroRNA在非特异性精神发育迟滞患者外周血中的异常表达  

Aberrant expression of microRNA in peripheral blood of non-specific mental retardation patients

作　　者：赵林[1] 张梁[2] 孙欣羊[3] 宋红涛[2] 范惠民[3] 张理义[4] 钟爱芳[5] 师征[5] 

机构地区：[1]徐州医学院研究生学院,徐州221002 [2]江苏大学医学院 [3]第二军医大学 [4]解放军第102医院全军心理疾病防治中心 [5]解放军第102医院检验科

出　　处：《中华行为医学与脑科学杂志》2014年第5期436-439,共4页Chinese Journal of Behavioral Medicine and Brain Science

摘　　要：目的 研究非特异性精神发育迟滞患者外周血中miRNA的异常表达.方法 采用基因芯片筛查技术对3例非特异性精神发育迟滞儿童和健康儿童外周静脉血的miRNA进行筛查,进行生物信息学分析,并选取其中10个感兴趣miRNA,运用实时定量PCR技术在34例非特异性精神发育迟滞患者与32例正常个体之间进一步验证芯片筛查的结果.结果 经筛查27个miRNA的表达差异具有统计学意义（上调或者下调倍数变化值≥2.0,P≤0.05）,其中22个表达上调,5个表达下调.经实时定量PCR验证miR-664a-5p,miR-1207-5p,miR-3141,miR-1228-5p,miR-4505在病例组（3.93±3.82,2.48±3.45,2.75±3.62,10.03±3.59,5.35±3.44）中表达较对照组（5.47±3.59,3.95±1.3.57,4.14±3.68,13.23±5.05,6.85±3.22）上调,差异有统计学意义（P＜0.05 ～ 0.01）.结论 多种miRNA在非特异性精神发育迟滞患者外周血中有异常表达.Objective To explore the differentially expressed miRNAs in peripheral blood firom the patients with non-specific mental retardation.Methods The miRNAs expression profilings in peripheral blood of 3 pairs in non-specific mental retardation patients and normal control were analyzed using microRNA array.10 miRNAs were selected according to the microRNA array results.The expression of these miRNAs were detected by quantitative RT-PCR in 34 non-specific mental retardation patients and 32 healthy individuals.Results There were 27 kinds of microRNA which had statistically different expression（FC ≥ 2.0,P≤ 0.05）,and among the above abnormal expressed miRNAs,22 were up-expressed （over-expression）,5 were down-expressed （under-expression）.The expression levels of miR-664a-5p,miR-1207-5p,miR-3141,miR-1228-5p and miR-4505 in non-specific mental retardation patients group （3.93 ± 3.82,2.48± 3.45,2.75 ± 3.62,10.03 ± 3.59,5.35 ± 3.44,respectively） were significantly up-expressed compared with the control group （5.47 ± 3.59,3.95 ± 1.3.57,4.14 ± 3.68,13.23 ± 5.05,6.85±3.22,P＜0.05-0.01）.Conclusion There are many differentially expressed miRNAs between the patients with non-specific mental retardation and healthy controls.

关 键 词：小分子RNA 非特异性精神发育迟滞 基因芯片 

分 类 号：R749.93[医药卫生—神经病学与精神病学]