人抗原RmRNA与蛋白和环氧合酶-2蛋白在卵巢浆液性肿瘤组织中的表达及其意义  

Expression of HuR mRNA and protein,COX-2 protein in ovarian serous carcinoma and its clinical significance

作　　者：成丽[1,2] 王敏[1] 王丹丹[1] 史聪[1] 双婷[1] 闫效宇[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]河北省保定市妇幼保健院

出　　处：《中国实用妇科与产科杂志》2014年第6期448-452,共5页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的探讨人抗原R(human antigen R,HuR)mRNA与蛋白和环氧合酶-2(COX-2)蛋白在卵巢浆液性肿瘤组织中的表达及其与卵巢浆液性癌临床病理生物学特征的关系。方法采用RT-PCR技术、免疫组化方法分别检测中国医科大学附属盛京医院2006年1月至2008年2月收集的31例卵巢浆液性癌组织标本、22例卵巢浆液性良性瘤组织标本中HuR mRNA与蛋白和COX-2蛋白的表达,并以8例正常卵巢组织作为对照组。结果 (1)HuR mRNA在卵巢浆液性癌中表达明显高于卵巢浆液性良性瘤和正常卵巢组织(P<0.05)。HuR mRNA的表达与卵巢浆液性癌细胞分化有关(P<0.05),与手术病理分期、淋巴结转移无关(P>0.05)。(2)HuR蛋白相对含量和胞浆表达阳性率在卵巢浆液性癌中的表达显著高于卵巢浆液性良性瘤和正常卵巢组织(P<0.05)。HuR蛋白相对含量和胞浆表达阳性率与卵巢浆液性癌细胞分化有关(P<0.05),与手术病理分期、淋巴结转移无关(P>0.05)。(3)COX-2蛋白相对含量与卵巢浆液性癌手术病理分期有关(P<0.05),与细胞分化、淋巴结转移无关(P>0.05)。依据31例卵巢浆液性癌中免疫组化结果半定量分析,经Spearman等级相关分析发现HuR与COX-2之间呈正相关(r=0.680,P=0.000)。结论卵巢浆液性肿瘤的发展与胞浆中HuR的过表达有一定相关性,HuR与COX-2蛋白在卵巢浆液性癌中的表达呈正相关。Objective To estimate the human antigen R mRNA and protein, COX-2 protein in ovarian serous tumors and understand their role in ovarian serous carcinoma genesis and progression and further to explore the relationship between HuR and COX-2. Methods The expression of HuR mRNA and protein, COX-2 protein were detected in 31 cases who underwent surgery for histologically verified ovarian serous carcinoma,22 cases verified ovarian serous benign tumor and 8 normal ovary tissues as control at the Department of Obstetrics and Gynecology, ShengJing Hospital, China Medical University between 2006 and 2008 by using RT-PCR,immunohistochemistry. Results （ 1 ） There is significant difference of expression of HuR mRNA between ovarian serous carcinoma and ovarian benign tumor,normal ovary tissue（P 〈 0. 05 ）. The expression of HuR mRNA was related with histological classification （ P 〈 0. 05 ） , however, which was not correlated with clinical staging,lymph node metastasis of ovarian serous carcinoma（ P 〉 0. 05 ）. （2）Immunohistochemical staining a- nalysis showed the relative content of HuR protein （IOD） and cytoplasmic HuR positve expression in ovarian serous carcinoma were statistically higher than that in ovarian benign serous tumor and normal ovary tissue （ P 〈 0. 05 ）. The relative content of HuR protein（IOD） and cytoplasmic HuR positive expression were related with histological classification（P 〈 0. 05 ） , however, which were not correlated with clinical staging,lymph node metastasis of ovarian serous carcinoma （P 〉 0. 05 ）. （ 3 ） There was a trend of correlation between cytoplasmic COX-2 and clinical staging（ P 〈 0. 05 ） , however, no significant association was found between cytoplasmic COX-2 and histological classification,lymph node metastasis of ovarian serous carcinoma（P 〉 0. 05）. According to Spearman rank correlation analysis, there is a positive correlation between HuR and COX-2 expression. Conclusion The overexpression of HuR is correlation with ova

关 键 词：人抗原R 卵巢浆液性肿瘤 环氧合酶-2 临床病理生物学特征 

分 类 号：R737.3[医药卫生—肿瘤]