16型人乳头状瘤病毒DNA及端粒酶在口腔粘膜病变中的表达  

Research on expression of human papillomavirus type 16 and telomerase in oral lesions

作　　者：帕提曼 张志愿[1] 曹俊[1] 张铃[2] 

机构地区：[1]上海第二医科大学口腔医学院口腔颌面外科,200011 [2]上海第二医科大学口腔医学院口腔内科,200011

出　　处：《中华口腔医学杂志》2001年第2期119-121,T003,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目!(39770 80 2 )

摘　　要：目的　探讨人乳头状瘤病毒 16型 (humanpapillomavirustype 16 ,HPV16 )感染与端粒酶活化在口腔粘膜癌变过程中的关系。方法　对口腔粘膜不同阶段病变的 82例标本进行HPV16DNA的聚合酶链反应检测及端粒酶活性催化亚单位 (humantelomerasereversetranscriptase,hTRT)mRNA的原位杂交检测。结果　正常口腔粘膜、上皮单纯性增生、上皮异常增生与鳞状细胞癌的HPV16感染率分别为 14 3 % (1/ 7)、42 0 % (3/ 7)、6 6 6 % (2 0 / 30 )及 92 1% (35 / 38) ;hTRTmRNA阳性表达率分别为0 0 % (0 / 7)、0 0 % (0 / 7)、30 0 % (9/ 30 )及 81 6 % (31/ 38)。hTRTmRNA与HPV16协同表达率为 6 7 0 %(5 5 / 82 )。结论　HPV16感染和端粒酶的激活与口腔粘膜恶变关系密切 。Objective To define a correlation between human papilllomavirus (HPV) type 16 and telomerase activity during the carcinogenesis of oral mucosa Methods HPV16 DNA and human telomerase reverse transcriptase (hTRT) mRNA were detected in 82 cases of paraffin embedded tissues including 7 cases of normal oral mucosa, 7 cases of hyperplasia lesions, 30 cases of oral dysplasia lesions and 38 cases of oral squamous cell carcinomas (OSCCs) by PCR and in situ hybridization (ISH) respectively Results HPV16DNA was positive in 14 3% (1/7) of normal oral mucosa, 42 9% (3/7) of hyperplasia lesions, 66 6% (20/30) of dysplasia lesions and 92 1% (35/38) of OSCCs hTRTmRNA was detectable in 30 0% (9/30) of oral dysplasia lesions and 81 6% (31/35) of OSCCs while normal oral mucosa and hyperplasia lesions were negative Expression of HPV16DNA and hTRTmRNA were co ordinate in 67 0% (55/82) cases Conclusions HPV16 infection may play an important role in carcinogenesis of oral mucosa by activation of telomerase

关 键 词：人乳头状瘤病毒 端粒酶 口腔鳞状细胞癌 口腔粘膜病变 DNA HPV16 

分 类 号：R781.5[医药卫生—口腔医学]