新疆阿勒泰羊钙蛋白酶抑制蛋白基因多态性与嫩度的关联性分析  

Association of Calpastatin Gene Polymorphisms with Meat Tenderness in Altay Sheep from Xinjiang

作　　者：田佳[1] 周卫东 马晓燕[1] 郑文新 高维明 迪拉热.沙塔尔 左晓佳 师帅 张蓉银 刘武军[1] 

机构地区：[1]新疆农业大学动物科学学院,新疆乌鲁木齐830052 [2]新疆维吾尔自治区种羊与羊毛羊绒质量安全监督检验中心,新疆乌鲁木齐830001

出　　处：《中国畜牧兽医》2014年第6期177-181,共5页China Animal Husbandry & Veterinary Medicine

基　　金：新疆维吾尔自治区高技术研究发展项目"新疆绵羊品种主要经济性状功能基因的鉴定"(201111115)

摘　　要：为研究钙蛋白酶抑制蛋白(calpastatin,CAST)基因多态性与新疆阿勒泰羊肉嫩度的关系,本试验以89只新疆阿勒泰羊为研究对象,运用PCR-SSCP方法对新疆阿勒泰羊CAST基因第1、2、21外显子进行多态性检测,采用DNA测序技术对结果进行测序,并与新疆阿勒泰羊肉嫩度进行关联性分析。结果显示,在第1和2外显子均未发现多态性,在第21外显子检测出AA、AB、BB 3种基因型,测序结果显示该处发生了A→G的突变。关联分析结果表明,该突变对阿勒泰羊肉嫩度有显著影响(P<0.05),初步推断AA基因型可能对阿勒泰羊肉嫩度有一定的改善作用。In this study, Ahay sheep was used for investigating the association between sheep tenderness and single nucleotide polymorphism markers on the calpastatin （CAST） gene. Its genetic polymorphism was detected using SSCP and DNA sequencing technology in exon 1, exon 2 and exon 21. However, only three genotypes AA, AB and BB were detected in exon 21, the sequencing result showed that the mutation of A into G, association analysis indicated that the mutation in exon 21 affected the meat tenderness markedly of Ahay sheep （P〈0.05）. In summary, AA genotype might have certain improvement effect on meat tenderness.

关 键 词：CAST基因 阿勒泰羊 嫩度 

分 类 号：S813.3[农业科学—畜牧学]