曲古菌素A诱导胃癌细胞下调蛋白的表达检测  

Detection of down regulation protein expression in gastric cancer cells induced by Trichostatin A

作　　者：李云龙[1] 李晓林[1] 崔武[1] 王浩[1] 田瑜[1] 曹志刚[1] 佟柏峰[1] 邹小明[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,150081

出　　处：《国际遗传学杂志》2014年第3期99-102,108,共5页International Journal of Genetics

基　　金：基金项目：教育部高等学校博士学科点专项科研基金（20102307120014）;哈尔滨市科技创新人才研究专项基金（2009RFQX016）

摘　　要：目的筛选曲古菌素A作用胃癌细胞系SGC-7901后明显表达下调的基因，检测其在临床胃癌组织中的表达水平。方法采用基因芯片技术，检测胃癌细胞系SGC-7901经过曲古菌素A干预后基因衷达的变化，筛选明显表达下凋的基因，应用免疫组化检测候选分子在胃癌及癌旁正常组织中的表达。结果胃癌细胞系SGC．7901经75ng／mL曲古菌素A作用48h后，Dickkopf related protein-1（DKK1）基因表达下调，差异具有统计学意义（P=0．0370）。免疫组化显示胃腺癌组织中DKK1基因表达明显高于周围正常胃组织，二者表达差异具有统计学意义（P=0．000）。结论DKK1基因在曲古菌素A作用胃癌细胞系SGC-7901后表达下调。DKK1基斟在胃腺癌组织中表达增高。Objective We investigated that the down-regulated genes were involved in Trichostatin A treatment in gastric cancer SGC-7901 cells. The expression of the candidate gene was detected in clinical gastricadenocarcinoma cases. Methods The changes of gene expression were detected in gastric cancer cell line SGC-7901 after Trichostatin A intervention by using gene chip technology. The down- regulated genes were screened and the expression was tested in gastric adenocarcinoma tissues by immunohistochemical analysis. Results Gastric cancer SGC-7901 cells with 75 ng/mL after 48 h of Triehostatin A treatment showed down-regulation of Dickkopf related protein-1 （ DKK 1 ） gene expression. The differences of DKKI expression were significant （P = 0. 037 0 ）. Immunohistochemistry result showed that the expression of DKKI in gastric adenocarcinoma was significantly tissues （P =0. 000 ）. Conclusion DKK1 expression was higher than that in the surrounding normal decreased in gastric cancer SGC-7901 cells ＇after Trichostatin A treatment. DKK1 is overexpressed in gastric adenocarcinoma tissue.

关 键 词：曲古菌素A SGC-7901 基因芯片 DICKKOPF related protein-1 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]