二甲基甲酰胺致心肌细胞氧化损伤的体外实验研究  

In vitro study of dimethylformamide induced oxidative damage to cardiomyocyte

作　　者：梁峰[1] 杨永坚[1] 向梅[1] 王娟[1] 

机构地区：[1]安徽医科大学公共卫生学院劳动卫生与环境卫生学系,安徽合肥230032

出　　处：《中华疾病控制杂志》2014年第6期549-552,共4页Chinese Journal of Disease Control & Prevention

摘　　要：目的探讨二甲基甲酰胺(dimethylformamide,DMF)对小鼠心肌细胞(H9c2)的氧化损伤作用及维生素C(vitamin C,VitC)的保护效应。方法使用不同剂量梯度DMF作用心肌细胞24 h、48 h、72 h,同时分别用含有DMF(100 mM)和0.025 mM、0.050 mM、0.100 mM、0.250 mM的VitC培养液培养心肌细胞24 h、48 h、72 h,采用试剂盒测定细胞内超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)的含量以观察细胞氧化应激状态和VitC的保护效应。结果不同浓度的DMF分别作用于心肌细胞24 h、48 h和72 h后使SOD和GSH含量降低,MDA含量升高,产生了明显脂质过氧化并呈现一定的剂量-时间效应关系。保护组VitC在低浓度(0.025 mM、0.050 mM、0.100 mM)时对DMF对心肌细胞的氧化损伤具有保护作用,使SOD和GSH含量升高,MDA降低。保护组VitC在高浓度(0.250 mM)时反而加重DMF对心肌细胞的氧化损伤。结论二甲基甲酰胺(DMF)对小鼠心肌细胞细胞(H9c2)产生了明显的氧化损伤,从而证实DMF所致的氧化应激是其引起细胞毒性的重要机制。小剂量VitC对DMF致心肌细胞的氧化损伤具有明显的保护作用。Objective To explore the oxidative stress effects of dimethylformamide （DMF） and the protective effect of VitC on the mouse myocardial cells （H9c2）. Methods The H9c2 cells were treated with different concentrations of DMF for 24 h,48 h and 72 h. Superoxide dismutase （SOD） and glutathione （GSH） and malondialdehyde （ MDA ） in su- pernatants were determined to evaluate the cytotoxicity and oxidative stress effects of DMF on H9c2 cells. The H9c2 cells were treated with different concentrations of VitC and DMF（ 100 mM） to observe the protective effect of VitC. The concentrations of VitC were 0. 025 mM, 0. 050 mM, 0. 100 mM and 0. 250 mM. Results After exposure of myocardial cells to different doses of for 24 h, 48 h and 72 h, SOD and GSH content decreased, MDA content increased, resulting in a dose- and time-dependent response manner. There was a significant protective effect for the oxidative damage of DMF when the H9c2 was exposed to the small dose of VitC（O. 025 raM, O. 050 mM and 0. 100 mM） , which reduced the MDA concentrations and increased GSH concentrations. However, VitC at high concentrations （0. 250 mM） actually worsened the effect of damage. Conclusions DMF can cause evidently oxidative damage in a dose-and time-dependent response manner. It suggested that the oxidative stress mechanism plays an important part in the cytotoxicity of DMF. The small dose of VitC have a significant protective effect on the oxidative damage of DMF to myocardial cells.

关 键 词：二甲基甲酰胺 肌细胞 心脏 抗坏血酸 

分 类 号：R135[医药卫生—劳动卫生]