ApoE 基因敲除对小鼠 B、T 淋巴细胞的影响  

The influence of B、T lymphocytes decreased in Apo E gene knockout mice

作　　者：石桂英[1] 张海涛[1] 张连峰[1] 白琳[1] 

机构地区：[1]中国医学科学院北京协和医学院实验动物研究所、卫生部人类疾病比较医学重点实验室,北京 100021

出　　处：《中国比较医学杂志》2014年第5期5-9,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然基金青年基金资助项目(81200256);协和青年基金资助项目(3332013040)

摘　　要：目的了解Apo E基因敲除对淋巴细胞的影响。方法用不同抗体标记Apo E基因敲除及同窝野生对照小鼠的外周血、脾脏、骨髓等细胞,应用流式细胞仪进行分析,比较两组之间的差异。结果对检测结果进行统计分析发现,与野生型小鼠相比,Apo E基因敲除小鼠的外周血、脾脏淋巴细胞、短期造血干细胞均有改变,骨髓前体B淋巴细胞、胸腺T淋巴细胞、长期造血干细胞等没有显著变化。结论 Apo E基因敲除后,小鼠外周血及脾脏B淋巴细胞减少,骨髓短期造血干细胞增加,但B及T淋巴细胞发育,以及骨髓长期造血干细胞、淋系共同祖细胞都没有显著变化。Objective To study the influence of Apo E gene knockout on the lymphocytes . Methods Cells from the peripheral blood, spleen and bone marrow of Apo E knockout and wildtype mice were stained with kinds of antibodies , and analyzed by flow cytometry .Results Compared with wildtype mice , significant differences were found in B and T lymphoctes in the peripheral blood and spleen , but there was no significant difference in pre B cells , T lymphocytes in the thymus and long term hematopoietic stem cell in the Apo E knockout mice .Conclusion Numbers of B lymphocytes decreased in the peripheral blood and spleen , but there was no significant difference in B , T lymphocytes development , and numbers of long term hematopoietic stem cell in Apo E gene knockout mice .

关 键 词：APO E基因敲除小鼠 流式细胞术 骨髓造血干细胞 

分 类 号：R332[医药卫生—人体生理学]