苦参碱对人肾母细胞瘤SK-NEP-1细胞趋化因子受体4表达的影响  

The effect of matrine on CXCR4 expression in SK-NEP-1 cells

作　　者：毛玲[1] 薛天阳[2] 许伟[2] 

机构地区：[1]徐州医学院,江苏徐州221004 [2]徐州医学院附属医院儿科,江苏徐州221002

出　　处：《临床儿科杂志》2014年第5期467-470,共4页Journal of Clinical Pediatrics

摘　　要：目的探讨苦参碱对体外培养人肾母细胞瘤SK-NEP-1细胞抑制增殖及诱导凋亡的作用。方法体外培养人肾母细胞瘤SK-NEP-1细胞分对照组,0.5 mg/ml、1.0 mg/ml、1.5 mg/ml苦参碱干预组,应用四甲基偶氮蓝比色法、流式细胞术检测苦参碱对SK-NEP-1细胞抑制率、凋亡率的影响,逆转录聚合酶链反应检测SK-NEP-1细胞的趋化因子受体4(CXCR4)mRNA表达。结果不同浓度苦参碱干预组及对照组SK-NEP-1细胞的抑制率、凋亡率以及CXCR4 mRNA表达差异均有统计学意义(P<0.01);随苦参碱浓度增加,SK-NEP-1细胞的抑制率和凋亡率呈上升趋势,而CXCR4 mRNA表达呈下降趋势,差异均有统计学意义(P<0.01)。结论苦参碱可以抑制SK-NEP-1细胞增殖并诱导凋亡,该作用可能与下调CXCR4的表达有关。Objectives To investigate the effects of matrine on the proliferation and apoptosis of SK-NEP-1 cells in vitro, and its possible mechanism. Methods Trials were divided into following groups：control group, 0.5, 1.0 and 1.5mg/ml of ma-trine intervention groups. The inhibition rate of SK-NEP-1 cells treated with different concentration of Matrine was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry （FCM）. RT-PCR analysis was employed to measure the PDCD4 mRNA expression. Results Matrine （final concentrations=0.5, 1.0 and 1.5mg/ml） could induce apoptosis and inhibit the growth of SK-NEP-1 cells. Compared with the controls without matrine treatment （8.81＆#177;3.71）%, the inhibition rates of SK-NEP-1 cells were （20.79 ＆#177; 6.20）%, （31.25 ＆#177; 5.07）%, and （51.15 ＆#177; 12.70）%, respectively;the apoptotic rates of SK-NEP-1 cells treated with different concentration of matrine were （13.67＆#177;0.78）%,（17.43＆#177;1.65）%and （20.80＆#177;1.54）%, respectively. Significant difference in the inhibition and apoptotic rates of SK-NEP-1 cells between each drug group and control group was observed（P〈0.05）, and the inhibition and apoptotic rates of SK-NEP-1 cells increased gradually with increased matrine concentration, thus exhibiting a dose-dependent effect（P〈0.05）. To the expression of CXCR4 mRNA,the grey levels of SK-NEP-1 cells treated with matrine intervention group （final concentrations=0.5, 1.0 and 1.5 mg/ml） were （0.720 ＆#177; 0.058）, （0.540 ＆#177; 0.095） and （0.307 ＆#177; 0.050）, respectively. The mRNA expression of CXCR4 was seen in SK-NEP-1 cells. Compared with control group, the expres-sion of CXCR4 mRNA was decreased significantly in matrine intervention group （P〈0.01）.There were significant difference in CXCR4 mRNA level among the SK-NEP-1 cells treated with 0.5,1.0,1.5mg/mL of matrine （P〈0.01）. Conclusions Matrine could induce apoptosis and inhibit the growth of SK-NEP-1 cells in a dos

关 键 词：人肾母细胞瘤SK-NEP-1细胞 苦参碱 趋化因子受体4 

分 类 号：R737.11[医药卫生—肿瘤]