乙醇预处理改善自酸蚀粘接剂的效前挥发初探  

Study of pretreatment with ethanol to compensate premature volatilization of self-etch adhesive system

作　　者：李红[1] 杨宏业[1] 黄翠[2] 邓东来[2] 

机构地区：[1]武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,430079 [2]武汉大学口腔医学院修复科,430079

出　　处：《中华口腔医学杂志》2014年第6期362-366,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81171010）

摘　　要：目的 研究乙醇预处理不同方式对效前挥发的自酸蚀粘接剂粘接性能的改善效果,以期为临床提供参考.方法 32颗无龋第三磨牙采用随机数字表法随机分为A组（丙酮基粘接剂,G-bond）和B组（乙醇基粘接剂,Clearfile S3bond）（每组16颗）;每组再按预处理方式不同分为4个亚组（每亚组4颗）：1亚组：使用未启封的粘接剂;2亚组：使用发生效前挥发的粘接剂;3亚组：依次使用梯度乙醇处理牙本质表面后再使用效前挥发的粘接剂;4亚组：无水乙醇处理牙本质表面后再使用效前挥发的粘接剂.粘接24 h后制备微拉伸试件（每亚组20个）,检测微拉伸强度,即粘接强度;使用体视显微镜和扫描电镜观察试件断面断裂模式.结果 A组内各亚组粘接强度差异有统计学意义（P＜0.05）;A2、A4组粘接强度[（26±12）和（27±7）MPa]均显著小于AI、A3组[（41±11）和（40±11） MPa] （P ＜0.05）,A2与A4组粘接强度差异无统计学意义（P＞0.05）,A1与A3组粘接强度差异无统计学意义（P＞0.05）.B组内各亚组粘接强度差异无统计学意义（P＞0.05）.两组内各亚组断裂模式差异均无统计学意义（P＞0.05）.扫描电镜显示,A组试件断裂模式以混合层底部断裂为主,各亚组树脂单体包裹胶原纤维的致密度不同,A1和A3组较其余两亚组致密.B组试件界面断裂模式以混合层顶部断裂为主.结论 效前挥发可降低丙酮基自酸蚀粘接剂的粘接强度,但对乙醇基自酸蚀粘接剂无明显影响;梯度乙醇预处理牙本质表面可有效改善丙酮基自酸蚀粘接剂的粘接效果.Objective To evaluate the effect of pretreatment with ethanol on dentin to compensate premature volatilization of self-etch adhesive system.Methods Thirty-two intact human molars were randomly divided into two groups using a table of random numbers （n =16）：A,an acetone-base adhesive （G-bond） and B,an ethanol-based adhesive（Clearfile S3 bond）.Then each group was randomly assigned into foursub groups （n =4）：group 1,no premature volatilization ; group 2,premature volatilization ; group 3,premature volatilization ＋ stepwise ethanol pretreatments; and group 4,premature volatilization ± absolute ethanol treatment.After composite resin building,microtensile bond strengths（MTBS） of each subgroup were then tested.Fracture modes were classified by stereomicroscopy and representative interface was observed by field-emission scanning electron microscopy （FE-SEM）.Results For adhesive A,there was significant difference on MTBS among different subgroups（P ＜0.05） ; the MTBS of group A2[（26 ± 12） MPa] and A4[（27 ±7） MPa] was lower than that of group A1 [（41 ± 11） MPa] and A3[（40 ± 11） MPa] （P ＜ 0.05）.No significant different was found between group A2 and A4 （P ＞ 0.05） ; neither between group A1 and A3 （P ＞ 0.05）.For adhesive B,there was no difference on MTBS among different subgroups （P ＞ 0.05）.FE-SEM showed that the main fracture mode was located at the bottom of the hybrid layer for adhesive A groups,the collagen fibrils were capsulated by resin monomers more densely in group A1 and group A3 in comparison with other two subgroups.For adhesive B groups,the main failure modes were at the top of the hybrid layer.Conclusions Premature volatilization can obviously decrease the bonding strength of acetone-base self-etch adhesives,but has no significant effect on ethanol-based self-etch adhesives.Dentin pretreatment with a series of increasing ethanol concentrations can effectively compensate the adverse effect of premature volatilization of acetone-base

关 键 词：牙本质粘结剂 乙醇 挥发作用 拉伸强度 

分 类 号：R783[医药卫生—口腔医学]