兔肺内肿瘤射频消融后残存肿瘤细胞增殖和新生血管变化  

Changes of proliferation and angiogenesis of residual tumor in rabbit lung after radiofrequency ablation

作　　者：马连君[1] 周乃康[1] 祁彦君 彭阳红 张书新 

机构地区：[1]解放军总医院胸外科,北京100853 [2]解放军第三〇九医院胸外科

出　　处：《中华医学杂志》2014年第21期1671-1673,共3页National Medical Journal of China

摘　　要：目的探讨兔肺内肿瘤射频消融（RFA）后残存肿瘤细胞增殖和新生血管的变化。方法采用组织块悬液注射法建立兔VX2肿瘤肺内种植模型，64只新西兰白兔随机分为对照组（n=10）和RFA组（n=54）。以控制电极展开范围、输出功率和治疗时间的方法，造成RFA后肿瘤残存。采用免疫组化法计算不同时间点残存肿瘤细胞Ki-67标记指数（Ki-67LI）和微血管密度（MVD）。结果对照组Ki-67LI为45．3％±2．1％。RFA组Ki-67LI在第1、3、5天分别为56．4％±3．4％、60．1％±4．1％和59．8％±2．4％，显著高于对照组（均P〈0．05）；在第7、9、14和21天分别为45．4％±2．0％、46．2％±3．4％、45．1％±4．4％和47．8％±3．9％，与对照组比较差异无统计学意义。对照组MVD为（28．9±2．9）条。RFA组MVD在第3、5、7天分别为（36．8±2．6）、（55．6±4．8）和（51．5±2．8）条，显著高于对照组（均P〈0．05）；在第1、9、14、21天分别为（27．0±2．8）、（29．2±3．2）、（30．0±2．8）和（28．8±3．1）条，与对照组比较差异无统计学意义。结论RFA后肺内残存肿瘤出现细胞增殖和新生血管一过性升高现象。Objective To observe the changes of proliferation and angiogenesis of residual tumor in rabbit lung after radiofrequeney ablation （RFA）. Methods The model of VX2 tumor in rabbit lung was established by injection of tissue block suspension. 64 New Zealand White rabbits bearing VX2 tumor were assigned randomly to the control group （n = 10） and the RFA group （n =48）. During the RFA procedure, residual tumors were achieved by controlling the range of electrode expanding, output power and treatment time. At several points of time, Ki-67 labeling index （Ki-67LI） and microvessel density （MVD） of the residual tumors were calculated by immunohistochemical detection. Results Ki-67LI of the control group was 45.3% ±2. 1%. Ki-67LI of the RFA group at the first, 3 and 5 day were 56.4% ±3.4%, 60. 1% ± 4. 1% and 59. 8% ±2.4% respectively, significantly higher than that of the control group; however, at the seventh, 9, 14 and 21 day, they were 45.4% ±2. 0%, 46. 2% ±3.4%, 45.1% ±4. 4% and 47.8% ± 3.9% respectively, no significant difference compared with the control group. The control group MVD was 28.9±2.9. MVD of the RFA group at third, 5 and 7 day were 36.8 ±2.6, 55.6 ±4.8 and 51.5 ±2.8 respectively, significantly higher than that of the control group; however, at the first, 9, 14 and 21 day were 27 ± 2. 8, 29. 2 ± 3.2, 30 ± 2. 8 and 28.8 ± 3.1 respectively, no significant difference compared with the control group. Conclusions The proliferation and angiogenesis of pulmonary residual tumor exhibit a transient increase phenomenon after RFA.

关 键 词：导管消融术 新生血管化 病理性 模型 动物 

分 类 号：R734.2[医药卫生—肿瘤]