博卡病毒的检测方法概述  

A Review of Detection Methods for Human Bocaviruses

作　　者：卢艳[1,2] 李丹地[2] 金玉[1] 段招军[2] 

机构地区：[1]南京医科大学附属南京儿童医院消化科,南京210000 [2]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《病毒学报》2014年第3期298-302,共5页Chinese Journal of Virology

摘　　要：自2005年博卡病毒发现以来，先后在呼吸道和肠道标本中检出博卡病毒1-4型。博卡病毒1主要与呼吸道感染相关，博卡病毒2-4型主要与肠道感染相关。博卡病毒感染具有典型的咳嗽、喘息、肺炎和腹泻等临床症状，然而由于博卡病毒缺乏合适的宿主细胞而难以培养限制其致病机理等相关研究。立体上皮细胞培养平台、反向遗传学和病毒宏基因组学作为新一代技术有望成为研究博卡病毒致病机理和发现新病毒的有力工具。本文针对博卡病毒已有的电镜、细胞培养、PCR和免疫学检测方法进行综述，以期为博卡病毒的深入研究提供方法参考。Human bocavirus （HBoV） 1-4 have been detected both in respiratory and stool samples since the first HBoV was discovered in 2005. HBoV-1 is mostly associated with respiratory infection, while HBoV 2-4 are usually associated with intestinal tract infection. A variety of signs and symptoms have been described in patients with HBoV infection, including cough, wheezing, pneumonia, and diarrhea, but the research on pathogenic mechanism of HBoV is limited because HBoV cannot be cultured in vitro due to the lack of appropriate host cells. Three-dimensional epithelial cell culture, reverse genetics, and viral metagenomics are identified as novel tools that may promote the research on pathogenic mechanism of HBoV and the discovery of new viruses. This review summaries currently available diagnostic approaches such as electron microscopy, cell culture, PCR, and immunoassay in order to provide a method reference for indepth research on HBoV.

关 键 词：博卡病毒 电镜 病毒分离培养 PCR 免疫方法 

分 类 号：R373.9[医药卫生—病原生物学]