实时荧光定量PCR检测胃癌SOX2的表达及其临床意义  

Expression and the clinicopathological significance of SOX2 in gastric cancer

作　　者：黄正接[1,2] 江龙[2] 尤俊[1,2] 曾岳岳[2] 吴炳霖[2] 陈百胜[2] 冯庆钊[1] 罗琪[1,2] 

机构地区：[1]厦门大学附属第一医院厦门市肿瘤中心肿瘤外科,福建厦门361003 [2]福建医科大学第一临床医学院,福建福州350004

出　　处：《癌变．畸变．突变》2014年第2期104-107,112,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：福建省科技计划重点项目(2010D026);福建省医学创新课题(2012-CXB-29);厦门市科技计划项目(3502Z20134011)

摘　　要：目的:探讨性别决定相关基因簇2(SOX2)表达与胃癌发生、发展和转移的关系及其临床意义。方法:用Trizol提取52例胃癌组织及对应正常胃黏膜组织的mRNA,并将其逆转录为cDNA。用实时荧光定量PCR方法检测SOX2 mRNA的表达,并分析SOX2基因的表达水平与患者临床病理指标的关系。结果:胃正常黏膜组织中SOX2 mRNA的表达是胃癌组织的2.24倍,两者差异有统计学意义(t=3.22,P=0.002 6)。高分化和中分化的胃癌组织中SOX2 mRNA表达量高于低分化的胃癌组织(t=3.09和2.87,P均<0.05),肿瘤浸润深度为T1+T2的胃癌组织中SOX2 mRNA表达量是T3+T4癌组织的2.57倍(t=2.16,P<0.05),淋巴结无转移组SOX2mRNA表达量是转移组的2.69倍(t=2.54,P<0.05)。与Ⅰ+Ⅱ期的胃癌组织比较,Ⅲ+Ⅳ期胃癌组织的SOX2 mRNA表达量明显降低(t=2.59,P=0.014)。SOX2 mRNA表达量与患者的性别、年龄无明显关系(P>0.05)。结论:SOX2 mRNA表达水平的降低与胃癌的发生、浸润和转移相关,有望成为胃癌诊断及预后判断的一个指标。OBJECTIVE：To investigate the expression of sex determining region Y-box 2（SOX2） in gastric cancer and explore the role of SOX2 in the development of gastric cancer. METHODS：Total RNA was extracted from 52 gastric cancer tissues and normal gastric mucosa tissues and reverse transcribed into cDNA. Real-time fluorescence quantitative RT-PCR was performed for evaluation of the expression of SOX2 mRNA in these tissues. RESULTS：SOX2 expression in well-differentiated and moderately differentiated gastric cancer tissues were higher than that of poorly differentiation（t=3.09 and t=2.87,P〈0.05）. Gastric cancer tissues showed higher SOX2 expression of invasion（T1＋T2） （t=2.16,P=0.037）,lymph node metastasis （t=2.54,P=0.017） andⅠ-Ⅱclinical stages （t=2.59,P=0.014） compared to deeper invasion（T3＋T4）,lymph node metastasis andⅢ-Ⅳclinical stages. There was no significant difference in SOX2 expression in the cancerous tissues of patients with different sex or age （P〉0.05）. CONCLUSION：Low SOX2 expression might be associated with the development,invasion and metastasis of gastric cancer and may potentially be used as marker for diagnosis and predicting the prognosis of gastric cancer.

关 键 词：胃肿瘤 性别决定相关基因簇2 实时荧光定量PCR 

分 类 号：R735.2[医药卫生—肿瘤]