卡托普利对大鼠肝星状细胞增殖的影响  

Effects of captlpril on proliferation of rat hepatic stellate cells

作　　者：李洵[1] 王爱民[1] 王军[1] 曹伟[1] 孟晓明[1] 

机构地区：[1]解放军252医院消化科,河北保定071000

出　　处：《中华实验外科杂志》2014年第6期1269-1270,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察血管紧张素Ⅱ（AngⅡ）、卡托普利对体外培养的肝星状细胞增殖的影响。方法采用肝星状细胞株HSC．T6作为活化的肝星状细胞的研究模型。采用噻唑蓝（MTT）法测定肝星状细胞的增殖。结果对照组A57。值为52．28±1．19；1×10^-5mol／LAngⅡ组A5，0值为74．81±3．61；1×10^-7mol／L AngⅡ组A570值为61．76±1．34；1×10^-9mol／LAngⅡ组A570值为58．12±1．29；1×10^-5 mol／L卡托普利＋AngⅡ组A570值为36．32±2．11；1×10^-7mol／L卡托普利＋AngⅡ组A570值为41．36±1．09；1×10^-9mol／L卡托普利＋AngⅠ组A570值为47．01±2．65。卡托普利＋AngⅡ组各浓度组A570值均明显低于对照组（P〈0．01），AngⅡ组各浓度组A570值均明显低于对照组（P〈0．01）。结论AngⅡ（1×10^-9～1×10^-5 mmol／L）可刺激肝星状细胞的增殖，而卡托普利则抑制肝星状细胞的增殖。Objective To investigate the effects of angiotensin Ⅱ （Ang Ⅱ ） and captlpril on proliferation of culured hepatic stellate cells in vivo. Methods HSC-T6 rat hepatic stellate cell line was chosen as the study model of the activated HSCs. Methyl thiazol tetrazolium （MTT） colorimetric assay was used to evaluate the proliferation of HSCs. Results A57o value of control groups was 52. 28 ± 1.19;A570 values of Ang Ⅱ groups （ 1 × 10^-5 mol/L,1 × 10^-7 tool/L,1 × 10^-9 mol/L） were 74. 81 ±3.61,61.76 ± 1.34,58.12 ± 1.29. A570 values of Captlpril ＋ Ang Ⅰ] groups （ 1 × 10^-5 mol/L, 1 × 10 ^-7 mol/L, 1 × 10^ -9 mol/L） were 36. 32 ± 2. 11,41.36 ± 1.09,47. 01 ± 2. 65. A570 value of Captlpril ＋ Ang Ⅱ groups was lower than that of control groups （ P 〈 0.01 ）. A570 value of Ang 11 groups was higher than that of control groups （ P 〈 0. 01 ）. Conclusion Angiotensin 1/ （ 1 × 10^-9 to 1 × 10^-5 mmo//L） stimulated proliferation of HSCs as demonstrated by MTT assay. Captlpril ＋ Ang Ⅱ had significantly inhibitory effect on growth of HSCs at the concentration of 1 × 10^-9 to 1 × 10^-5 mmol/L.

关 键 词：血管紧张素Ⅱ 卡托普利 肝星状细胞 增殖 

分 类 号：R575.2[医药卫生—消化系统]