奥沙利铂节律化疗对小鼠胃癌的抑制作用及其机制  

Inhibitory effect of low-dose metronomic Oxaliplatin chemotherapy on gastric cancer growth in mice and the action mechanism

作　　者：高玉宝[1] 黄建朋[3] 徐英[2] 

机构地区：[1]丹江口市第一医院普外科,湖北442700 [2]丹江口市第一医院皮肤科,湖北442700 [3]湖北医药学院附属人民医院胃肠外科

出　　处：《中华实验外科杂志》2014年第6期1277-1278,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察奥沙利铂低剂量节律化疗（LDM）在小鼠胃癌中的抑瘤效果、不良反应并探讨其机制。方法BALB／c—nu小鼠皮下接种胃癌细胞株MGC-803细胞，随机分为3组，每组20只。节律组：奥沙利铂1mg／（kg·d）腹腔注入，持续2周；最大耐受剂量（MTD）组：1次腹腔注入奥沙利铂14mg／（kg·d）；对照组：生理盐水0．2ml每日腹腔注入，持续2周。接种后第15天处死小鼠，完整剥离皮下瘤结节，计算抑瘤率，行组织学观察并采用免疫组织化学法检测肿瘤内微血管密度（MVD）、血管内皮生长因子（VEGF）表达。结果节律组及MTD组抑瘤率分别为59．6％、39．4％，差异有统计学意义（P〈0．05），节律组肿瘤组织可见大量的细胞变性坏死，MTD组少见。节律组肿瘤组织MVD及VEGF蛋白表达较MTD组显著减少（P〈0．01）。结论奥沙利铂低剂量节律化疗可显著抑制BALB／c—nu小鼠胃癌肿瘤的生长，其机制可能为奥沙利铂下调VEGF表达，抑制肿瘤血管生成，进而抑制肿瘤生长。Objective To explore the inhibitory effect of low-dose metronomic Oxaliplatin chemotherapy on gastric cancer growth in mice and the action mechansim. Methods Sixty BALB/e-nu mice were subcutaneously implanted with MGC-803 cells, and evenly divided into three groups ： Low-dose metronomic （LDM） group, Oxaliplatin 1 mg/（kg·d）, once a day for two weeks; Maximum tolerated dose （MTD） group： Oxaliplatin 14 mg/（kg·d） single dose, and intraperitoneal administration; control group： intraperitoneal injection of 1.2 ml normal saline, once every day for 2 weeks. The mice were killed on the day 15. The tumors were then weighed, and tumor mierovessel density （MVD） and the expression of vascular endothelial growth factor （VEGF） were detected by immunohistochemieal staining. Results The cancer suppressive rate in LDM and MTD groups was 59.6% and 39.4% respectively. The expression of VEGF and MVD was significantly lower in LDM group than in MTD group （ P 〈 0. 05 ）. Conclusion Angiogenesis is significantly inhibited in mice receiving LDM treatment, which may be correlated with the low wexpression of VEGF in the tumors.

关 键 词：胃癌 奥沙利铂 化疗 血管生成 

分 类 号：R735.2[医药卫生—肿瘤]