血管生成素-1对大鼠局灶性脑缺血再灌注后血脑屏障的保护作用  

The protective role of angiopoietin-1 in blood brain barrier permeability after cerebral ischemia reperfusion in rats

作　　者：高轩[1] 李洪亮[2] 李永秋[1] 鲁琳[1] 赵明艳[1] 

机构地区：[1]河北省唐山工人医院神经内科,063000 [2]江苏省淮安市第三人民医院神经科

出　　处：《中华实验外科杂志》2014年第6期1289-1291,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察颈总动脉注射血管生成素-1（Ang-1）对大鼠局灶性脑缺血再灌注损伤后血脑屏障的保护作用。方法大鼠30只，采用大脑中动脉线栓法建立大鼠局灶性脑缺血再灌注损伤模型，按照随机数字表法分为3组，假手术组、生理盐水组和Ang-1治疗组，每组各10只。再灌注后48h处死大鼠，分别进行大鼠脑组织含水量、血脑屏障通透性、脑梗塞体积检测以及大鼠神经功能评分。结果再灌注48h后，Ang-1治疗组大鼠脑组织含水量为（68．69±4．46）％，显著低于生理盐水组[（79．08±4．12）％，t=4．33，P〈0．05]，和假手术组比较差异统计学意义[（64．37±3．28）％，t=1．387，P〉0．05]。假手术组、生理盐水组和Ang-1治疗组大鼠缺血区脑组织Evans blue（EB）含量分别为（87．40±9．42）μg／g组织、（381．40±22．30）μg／g组织和（130．90±16．80）μs／g组织。Ang-1治疗组EB含量和与生理盐水组比较差异有统计学意义（t=15．12，P〈0．01），与假手术组比较差异无统计学意义（t=0．934，p〉0．05）。Ang-1治疗组大鼠脑梗塞体积为（0．119±0．022）cm^3，显著低于生理盐水组[（0．407±0．036）cm^3，t=6．327，P〈0．01]，和假手术组比较差异无统计学意义[（0．104±0．011）cm^3，t=1．099，P〉0．05]。假手术组、生理盐水组和Ang-1治疗组神经功能损伤评分分别为（0．79±0．11）、（2．47±0．34）和（1．83±0．29）分，Ang-1治疗组神经功能损伤评和与生理盐水组比较差异有统计学意义（t=4．37，P〈0．05），与假手术组比较差异有统计学意义（t=2．31，P〈0．05）。结论Ang-1可以降低大鼠局灶性脑缺血再灌注后血脑屏障的通透性，对脑缺血再灌注损伤具有保护作用。Objective To investigate the protective role of angiopoietin-1 （Ang-1） in blood brain barrier permeability after cerebral ischemia reperfusion in rats. Methods Rat cerebral ischemia reperfusion was established by middle cerebral artery occlusion （MCAO） and randomly divided into 3 groups： sham operated group, saline group and angiopoietin-1 treatment group （n = 10）. The cerebral water content, brain barrier permeability, infarct volume and the neurological deficits scores were measured 48 h after reperfusion. Results 48 h after reperfusion, the cerebral water content of Ang-1 group was （68.69 ± 4. 46） %, signifi- cantly lower than that of saline group [ （79.08 ± 4. 12） %, t = 4. 33, P 〈 0. 05 ], and similar to the sham group [ （64. 37 ± 3. 28 ） %, t = 1. 387, P 〉 0. 05 ]. The evans blue （ EB ） content of three groups were （87.40 ± 9. 42） p.g/g tissue, （381.40±22. 30） μg/g tissue and （ 130. 90± 16. 80） μg/g tissue, respectively. The EB content of Ang-1 group was significantly lower than that of saline group （t = 15.12, P 〈 0. 01 ） and similar to the sham group （ t = 0. 934, P 〉 0. 05 ）. The infarct volume of Ang-1 group was （0. 119 ± 0. 022） cm^3, significantly lower than that of saline group [ （0. 407 ± 0. 036） cm^3, t = 6. 327, P 〈 0. 01 ] and similar to the sham group [ （0. 104 ± 0. 011 ） cm^3, t = 1. 099, P 〉 0. 05 ]. The neurological deficits scores of three groups were （0. 79 ± 0. 11 ）, （2.47 ± 0. 34） and （ 1.83 ± 0. 29 ）, respectively. The neurological deficits scores of Ang-1 group was significantly lower than that of saline group （t = 4. 37, P〈0.05） and similar to the sham group （t=2.31, P〈0.05）. Conclusion Ang-1 has a protective effect on the permeability of blood brain barrier after cerebral ischemia-reperfusion.

关 键 词：血管生成素-1 脑缺血 再灌注损伤 血脑屏障 

分 类 号：R743.3[医药卫生—神经病学与精神病学]