结直肠癌患者差异蛋白质组学研究  

Differential proteomic study of colorectal cancer

作　　者：黄汉源[1] 来伟[2] 张旸[2] 曾献清 陈志坚[1] 李定文[1] 褚忠华[2] 

机构地区：[1]广东省五华县人民医院普外科,514400 [2]中山大学孙逸仙纪念医院

出　　处：《中华实验外科杂志》2014年第6期1328-1330,共3页Chinese Journal of Experimental Surgery

基　　金：吴阶平医学基金会临床科研专项基金资助项目（320.6750.12376）;广东省梅州市科技计划项目（20128104）

摘　　要：目的应用差异蛋白质组学方法筛选结直肠癌组织和对应正常组织的差异蛋白表达。方法采用双向凝胶电泳（2-DE）分离23例结直肠癌患者和对应正常黏膜组织的总蛋白，从中选取差异表达蛋白质位点，通过基质辅助激光解吸电离飞行时间串联质谱（MALDI—TOF—TOF—MS）鉴定差异表达的蛋白质；然后采用Western blot验证蛋白质组学分析的结果。结果通过2-DE图谱分析发现8个差异蛋白质斑点，质谱鉴定出6种蛋白质，在结直肠癌患者组织中高表达的有3个，其余3个在正常组织黏膜中低表达。Western blot对表达增高的翻译调节的肿瘤蛋白（TCTP）进行检测，结果与蛋白质组学分析结果一致。结论蛋白质组学研究是筛选结直肠癌组织中特异性标志物的有效手段。Objective To screen the tissue proteins associated with the colorectal carcinoma using a comparative proteomie approach. Methods Two-dimensional electrophoresis was applied to separate the proteins in eolorectal cancer specimens and paired normal group from 23 colorectal cancer patients. The proteins with statistically significant differential expression between the colorectal carcinoma and normal groups were identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry （ MALDI-TOF-TOF-MS）. Western blotting was performed to examine the differential expression of the candidate proteins. Results Eight protein spots were found as differentially expressed proteins, in which 6 proteins were identified successfully. Among the identified proteins, there were 3 over-expressed and 3 under-expressed proteins in colorectal cancer tissues as compared with normal group. Translationally controlled tumor protein （TCTP） was found to be over-expressed in colorectal cancer tissues in comparison with the normal group, as confirmed by Western blotting. Conclusion Comparative proteomics analysis provides an effective approach to screen the special biological markers for colorectal cancer patients.

关 键 词：蛋白质组学 双向凝胶电泳 结直肠癌 

分 类 号：R735.34[医药卫生—肿瘤]