应用液相色谱-飞行时间质谱技术对肺癌患者血清代谢组学的研究  

Liquid chromatography-tandem mass spectrometry-based research on serum metabonomics of lung cancer patients

作　　者：陈莹蓉[1] 马志红[1] 李爱英[2] 李鸿伟[2] 王斌[3] 钟婧[1] 闵丽姗[1] 戴利成[1] 

机构地区：[1]浙江省湖州市中心医院、湖州市分子医学重点实验室,313000 [2]浙江省湖州市中心医院心胸外科,313000 [3]浙江省湖州市中心医院呼吸内科,313000

出　　处：《中华实验外科杂志》2014年第6期1356-1358,共3页Chinese Journal of Experimental Surgery

基　　金：浙江省科技厅一般科研计划公益技术应用研究项目（2011C23131）

摘　　要：目的应用代谢组学技术分析肺癌患者和正常人群血清的代谢物质。方法运用高效液相-四级杆-飞行时间质谱（LC—Q—TOF／MS）联用技术对30例肺癌患者与30名正常人血清进行研究。采用正交最小二乘分析法（OPLS）进行建模，运用两样本t检验寻求两组间差异性代谢物。结果多变量统计结果显示肺癌患者和正常对照人群的代谢谱有明显差异，根据t检验结果寻找到血清中相关的差异代谢物36种。结论建立了利用LC—Q—TOF／MS技术进行肺癌血清代谢组学研究的方法。Objective To explore the differences in serum metabolism between patients with lung cancer and healthy people using the technology of metabolomies. Methods Serum samples from 30 lung cancer patients and 30 healthy people were analyzed by high performance liquid chromatography-quadrupole time-of-flight mass spectrometry （HPLC-Q-TOF/MS）. Orthogonal to partial least squares discriminant a nalysis was performed for modeling. Two sample t-test was used to identify differences in metabolite concentrations. Results Multivariate statistical analysis yielded a significant distinction in the metabolic profiles between lung cancer patients and healthy people. The t-test results indicated a total of 36 metabolites in the serum with statistically significant differenees. Conclusion The method of lung cancer serum metabolomics study has been constructed using the technology of HPLC-Q-TOF/MS.

关 键 词：肺癌 高效液相色谱-四极杆-飞行时间质谱 代谢组学 

分 类 号：R734.2[医药卫生—肿瘤]