基于离心染色全血分析系统光路及算法设计  

Optical path and algorithm design of complete blood analysis system based on centrifugation and staining techniques

作　　者：段浩[1] 陈锋[1] 李抄[1] 顾彪[1] 吴太虎[1] 

机构地区：[1]军事医学科学院卫生装备研究所,天津300161

出　　处：《仪器仪表学报》2014年第6期1315-1324,共10页Chinese Journal of Scientific Instrument

基　　金：国家十二五重大专项(AWS11Z005-4);国家科技重大专项(2012ZX10004801)资助项目

摘　　要：针对现有湿式全血分析仪在环境适用性方面存在的不足以及干式分析仪在信号提取方式上的缺点,提出一种全新的基于毛细管离心和细胞染色技术的干式血液分析系统设计,以满足复杂环境下血液的快速分析,主要阐述了新型干式全血分析系统信号提取的光路设计和自适应处理算法的原理。所设计分析系统在红、蓝光源照射下采用彩色线阵CCD(电荷耦合器件)提取离心后染色毛细管一维图像,通过算法处理提取边缘信息,最终由层厚转化为血液成分参数,并实现白细胞两分类。实验结果表明,相比于同类干式分析仪器,该系统和湿式测量结果一致性较高,重复性更好,多复杂环境适应性大大增强,所设计的一维信号处理算法具有良好的自适应性和通用性。Aiming at the deficiency of existing wet-type hematology analyzers in environmental applicability and the shortcoming of dry- type hematology analyzers in signal extraction method, we propose the novel design of a dry-type complete blood analysis system based on capillary eentrifugation and eel1 staining techniques to satisfy the requirement of rapid blood analysis in complex environment. The principles of the optical path design for signal extraction and the adaptive processing algorithm are introduced in the paper. Under the irradiation of red and blue light sources, the one-dimensional images of the centrifuged staining capillaries can be obtained usinl g a color linear CCD （charge coupled device）. The proposed algorithm is used to extract the edge information and determine the layer thicknesses, from which the blood component parameters can be determined and white blood calls can be classified into two categories. Experiment results show that compared with the same kind dry-type analyzer, the test result of the proposed system is highly consistent with that of the wet- type analyzer and has better repeatability. In addition, the adaptability of the analyzer to multiple complex environments has been greatly strengthened and the designed one-dimensional signal processing algorithm shows good performance both in adaptability and generality.

关 键 词：离心染色 信号提取 算法设计 重复性 自适应 

分 类 号：TH776[机械工程—仪器科学与技术]